Project description:Setaria viridis, the wild ancestor of millet, exhibits strong repression of crown root growth in drought. We compare in gene expression in the S. viridis crown between drought vs watered treatments.
2016-07-22 | GSE78054 | GEO
Project description:BAC pool sequencing of broomcorn millet
Project description:Setaria viridis, the wild ancestor of millet, exhibits strong repression of crown root growth in drought. We compare in gene expression in the S. viridis crown between drought vs watered treatments. RNA from Lower region (crown) or Upper region (stem) of watered (W) or drought (D) treated Setaria plants were harvested at 6 or 9 days after sowing; there are 8 samples per biological repeat, 3 biological repeats.
Project description:Foxtail millet (Setaria italica L. P. Beauv) has been considered as a tractable model crop in recent years due to its short growing cycle, lower repetitive DNA, inbreeding nature, small diploid genome, and outstanding abiotic stress-tolerance characteristics. With modern agriculture often facing various adversities, it’s urgent to dissect the mechanisms of how foxtail millet responds and adapts to drought and stress on the proteomic-level.
Project description:Pearl millet [Pennisetum glaucum (L.) R.Br] is the fifth most important cereal crop next to rice, wheat, maize, and sorghum. It is cultivated especially by small holder farmers in arid and semi-arid regions because of its drought resistance. However, the molecular mechanisms during drought stress in Pennisetum remain elusive. In the present study we have used a shotgun proteomics approach (GEL-LC-Orbitrap-MS) for identification and quantification of proteins from different tissues (root, seed and leaf) under drought and control conditions. Plants were grown in a tube system to survey root growth under drought stress. The water content was measured in the upper and the lower part of the tube using soil moisture sensors. Under drought stress root elongation was observed. Measurement of stomatal conductance showed a clear response to drought stress. For proteomics measurements root, leaf and seed tissues were harvested. In total 2281 proteins were identified, 1095 in root, 1299 in seed, and 1208 in leaf in both stress and control conditions.
Project description:The marker- and genome-based development of drought resistant and high yield cereal crops is the most pressing activity in a constantly more stress- and harmful environment for plant productivity. Genome-based assisted breeding is only capable to cover 30-40 % of phenotypic variance according to the most recent GWAS studies. There are many processes which are not predictable by genome information, especially protein translation and activity which is crucial for phenotypic responses and survival of the plants under severe stresses. Therefore, we present comparative proteomic and physiological analysis under drought stress in two of the most important staple food crops pearl millet and wheat thereby representing C4 and C3 plants. Here, we have selected contrasting genotypes, and performed a large-scale comparative analysis from the molecular to the phenotypic level under drought stress. We were able to establish molecular-physiological phenotypes for: 1. Stay green protein signature in contrasting pearl millet genotypes which is highly correlated to the physiological data, in the submitted manuscript. 2. No clear indication of stay green proteome signatures in contrasting wheat genotypes but instead differential senescence proteome signatures not capable to cope with similar drought stress. These mechanisms are decisive for drought resistance and yield/grain filling under stress conditions and for the first time these physiological phenotypes (seed yield, root growth, and photosynthesis) are directly linked to the molecular proteomic phenotype. We think these results are of broad importance for the scientific community. This study demonstrates the enormous molecular and phenotypic plasticity because the selected genotypes represent the extreme points of stress adaptation and yield protection.
Project description:To investigate the different drought response between the different maize lines, we screened drought-tolerant line W9706 and drought-susceptible line B73 to compare the transcriptome
Project description:Transcriptional profiling of rust-infected pearl millet seedlings over time [0h, 20h, 5d and 8d post infection (pi)]. Keywords: Time course, Stress response
