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These data describe bulk RNAsequencing in wild-type cerbreal organoids sampled across a time series

ABSTRACT: These data describe bulk RNAsequencing in wild-type cerbreal organoids sampled across a time series

PROVIDER: PRJNA751896 | ENA |

REPOSITORIES: ENA

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These data describe bulk RNAsequencing in wild-type cerbreal organoids sampled across a time series

Project description:Organoid transcriptomes were mapped onto brain development trajectories using bulk RNA sequencing (RNAseq) at seven successive developmental times from day-17 (2 weeks) today-158 (11 weeks)

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RNAsequencing of the ventral rhombomere 1 of the developing mouse [bulk RNA-seq]

Project description:Sequencing of the mouse ventral rhombomere 1 tissues and cells to characterize the Tal1-dependent and -independent neuronal precursor groups. The data allowed to describe the cell types in the R1 brain region, and map gene expression dynamics during bifurcation of the GABAergic and glutamatergic lineages. Our results provide a framework for understanding the development of cellular diversity in the tegmental nuclei.

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Single-Cell RNAsequencing of Human Naïve Pluripotent Stem Cells to Trophoblast Stem Cells with and without EZH2 inhibition.

Project description:Single-Cell RNAsequencing of the conversion of naïve iCas9 Sigma hiPSC line 1 (iCSIG-1 hiPSCs) into trophoblast stem cells with and without EZH2i (Abcam ab146152 UNC1999, EZH2 methyltransferase inhibitor) during 4 days preconversion and during 4 days during conversion (total of 8 days with or without PRC2i).

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Project description:<p>Organic carbon in seawater plays a significant role in the global carbon cycle. The concentration and composition of dissolved organic carbon, operationally defined in this project as organic carbon that passes through a 0.2 µm filter, reflect the actions of the biological community and chemical reactions that occur in seawater. Here, we repeatedly sampled the oligotrophic northwest Sargasso Sea in the vicinity of the Bermuda Atlantic Time-Series Study site (BATS) to quantitatively follow select known compounds within the pool of dissolved organic matter in the upper 1000 meters of the water column over a four-year period. Metabolite concentrations revealed patterns with depth and time with most metabolites showing surface enrichment and lower concentrations with depth. Select metabolites had a pattern of increased and decreased concentrations throughout the year, which was observed in each of the years sampled. Vitamins, including pantothenic acid, biotin, and riboflavin, presented annual increases in the winter period when mixed layer depths are deepest. Light-sensitive riboflavin also showed significant decreases during daylight hours under diel sampling. The metabolites examined in this study are all components of central carbon metabolism. By examining these metabolites at finer resolution and in a relatively long time series, we have clues on microbial actions in marine systems, data which are fundamental to understanding the chemical response of marine systems to future changes in climate.</p>

2022-02-09 | MTBLS2356 | MetaboLights

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