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Disentangling glial diversity in peripheral nerves at single-nuclei resolution [single-cell and single-nuclei RNA-seq]

ABSTRACT: Disentangling glial diversity in peripheral nerves at single-nuclei resolution [single-cell and single-nuclei RNA-seq]

PROVIDER: PRJNA754445 | ENA |

REPOSITORIES: ENA

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Disentangling glial diversity in peripheral nerves at single-nuclei resolution

Project description:Disentangling glial diversity in peripheral nerves at single-nuclei resolution

| PRJNA754444 | ENA

Disentangling glial diversity in peripheral nerves at single-nuclei resolution [bulk and RiboTag RNA-seq]

Project description:Disentangling glial diversity in peripheral nerves at single-nuclei resolution [bulk and RiboTag RNA-seq]

| PRJNA753716 | ENA

Disentangling glial diversity in peripheral nerves at single-nuclei resolution [single-cell and single-nuclei RNA-seq]

Project description:The peripheral nerve contains diverse cell types that support its proper function and maintenance. We analyzed multiple peripheral nerves using single-nuclei RNA-sequencing (snRNA-seq), which allowed us to circumvent difficulties encountered in analyzing cells with complex morphologies via conventional single cell methods. The resultant mouse peripheral nerve cell atlas highlights a diversity of cell types, including multiple subtypes of SCs, immune cells, and stromal cells. We identified a distinct myelinating-SC subtype that expresses Cldn14, Adamtsl1, and Pmp2 and preferentially ensheathes motor axons. The number of these motor-associated, Pmp2+ SCs is reduced in both ALS SOD1G93A mouse model and human ALS nerve samples. Our findings reveal the diversity of SCs and other cell types in peripheral nerve and serve as a reference for future studies of nerve biology and disease.

2021-12-06 | GSE182098 | GEO

Disentangling glial diversity in peripheral nerves at single-nuclei resolution [bulk and RiboTag RNA-seq]

2021-12-06 | GSE181858 | GEO

Disentangling glial diversity in peripheral nerves at single-nuclei resolution

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-12-06 | GSE182099 | GEO

Ambient RNA analysis of single-nuclei RNA-Seq data from the frontal cortex of C57BL/6J mice

Project description:Contamination of ambient RNA was found in single-nuclei RNA-Seq data of mouse frontal cortex. CellBender plus subcluster cleaning selectively removed ambient RNA signature from glial cell types.

2022-10-19 | GSE198640 | GEO

Placental infection single-cell and single-nuclei maps

Project description:This resource comprises a single-cell multi-lineage map of first trimester infected placental cells. We have included data from both uninfected cells and cells infected with three pathogens known to cause maternal and fetal disorders: Plasmodium falciparum, Listeria monocytogenes, and Toxoplasma gondii. We also generated single-nuclei map of infected trophoblasts and their corresponding controls. Furthermore, we created a single-nuclei reference dataset containing information from uninfected primary placental organoids as well as organoids infected with P. falciparum. Additionally, we conducted sequencing at a single-cell level for P. falciparum parasites that were bound to the placenta (pf_b), parasites unbound to the placenta (pf_nb), and parasites that were cultured in vitro (pf_iv).

2024-03-01 | E-MTAB-12795 | biostudies-arrayexpress

Single-nuclei profiling of aged skeletal muscle

Project description:Single-nuclei profiling of aged skeletal muscle

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Single-nuclei RNAseq (NucSeq) of human eyes

Project description:Single-nuclei RNAseq (NucSeq) of human eyes

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Single nuclei RNA-seq from adult mouse Hippocampus

Project description:We report RNA-seq of single nuclei isolated from the adult C57BL/6 male mouse Hippocampus region. Majority of the nuclei were isolated from 12 weeks old mice (4 different animal), with an additional set of nuclei from 3 months and 2 years old animals. In addition a set of GFP labeled nuclei driven by a VGAT promoter . Microdissections of dentate gyrus, CA1 and CA2/3 regions of the Hippocampus were placed into ice-cold RNA-later for fixation and stored at 4âc overnight, then stored in -80âc. Nuclei were isolated by sucrose gradient centrifugation and kept on ice until sorting using Fluorescence Activated Cell Sorting (FACS) into 96 well plates containing RNA lysis buffer. Single nucleus RNA was first purified then derived cDNA libraries were generated following a modified Smart-seq2 protocol. For VGAT nuclei: high titer AAV1/2 of pAAV-EF1a-DIO-EYFP-KASH-WPRE-hGH-polyA was injected into dorsal and/or ventral Hippocampus, animals were sacrificed two weeks after injections, and GFP labeled nuclei were sorted into plates and processed as described above.

2016-08-16 | E-GEOD-85721 | biostudies-arrayexpress

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