Project description:This study is a time course of growth induction (0, 12, 24, 48, and 72 hrs) following the breaking of paradormancy in underground buds of the perennial weed leafy spurge (Euphorbia esula). Keywords: Time course, growth induction, apical dominance, paradormancy, root buds, leafy spurge
Project description:The low-resolution structure of a nucleotide pyrophosphatase/phosphodiesterase from Euphorbia characias latex (ELNPP) has been determined in solution by means of Small Angle X-ray Scattering (SAXS). To improve the structural resolution of ELNPP a partial sequencing after proteolytic cleavage of the protein was performed. Protein digestion followed by high-resolution HPLC-ESI-MS/MS analysis allowed us to identify two different peptides of 82 and 41 amino acids, respectively. These sequences exhibit a high degree of identity with other NPPs predicted sequences from several higher plants including Malus domestica, Morus notabilis, Ricinus communis, and Triticum aestivum. In particular, Triticum aestivum NPP is the protein with the highest identity level (98 identical positions) towards the obtained fragments of ELNPP. From the alignment with the entire sequence of TaNPP, the two obtained fragments contain some known conserved residues belonging to the catalytic domain of the mammalian NPP family enzymes.
