Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq

ABSTRACT: Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq

PROVIDER: PRJNA764231 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series1: no enrichment]

Project description:DNA methylation plays important roles in foreign DNA defense, mismatch repair, and gene regulation in prokaryotic genomes. Existing methods for DNA methylation detection using next-generation sequencing (NGS) are incapable of simultaneously detecting multiple types of DNA methylation. Here, we present nitrite treatment followed by sequencing (NT-seq), a sequencing method to simultaneously detect adenine and cytosine methylation. We demonstrated that NT-seq reliably detects three types of methylation motifs in E. coli and H. pylori genomes. We further applied NT-seq to a microbial community standard for de novo methylation motif discovery. Finally, by coupling methyl DNA immunoprecipitation and NT-seq (DIP-NT-seq), we showed that 6mA could be accurately mapped at single-base resolution in the bacterial and eukaryotic genomes. NT-seq thus provides a simple and reliable solution for detecting multiple types of DNA methylations.

2022-05-12 | GSE184372 | GEO

Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series2: 6mA DIP enrichment]

2022-05-12 | GSE184373 | GEO

Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series1: no enrichment]

Project description:Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series1: no enrichment]

| PRJNA764234 | ENA

Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series2: 6mA DIP enrichment]

Project description:Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq [series2: 6mA DIP enrichment]

| PRJNA764233 | ENA

Bimodal Adenine and Cytosine CRISPR Base Editing on DNA

Project description:Adenine and cytosine base editors (ABEs and CBEs) represent a new genome editing technology that allows the programmable installation of A-to-G or C-to-T alterations on DNA. We engineered Streptococcus pyogenes Cas9-based adenine and cytosine base editor (SpACE) that enables efficient simultaneous introduction of A-to-G and C-to-T substitutions in the same base editing window on DNA.

2020-07-02 | GSE137411 | GEO

Simultaneous Detection of DNA Adenine and Cytosine Methylation using NT-seq

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-05-12 | GSE184374 | GEO

Transcriptome-wide effects of cytosine and adenine deaminases on gene expression induced by base editors

Project description:We used RNA-sequencing data to explore the transcriptome-wide effects of cytosine and adenine deaminases on gene expression induced by base editors.

2021-06-01 | GSE158693 | GEO

Bimodal Adenine and Cytosine CRISPR Base Editing on DNA

Project description:Bimodal Adenine and Cytosine CRISPR Base Editing on DNA

| PRJNA565401 | ENA

Precise adenine base editors that exhibit minimized cytosine catalysis

Project description:Precise adenine base editors that exhibit minimized cytosine catalysis

| PRJNA693459 | ENA

CRISPR adenine and cytosine base editors with reduced RNA off-target activities

Project description:CRISPR adenine and cytosine base editors with reduced RNA off-target activities

| PRJNA533102 | ENA