Project description:Genome wide DNA methylation profiling of 4 cell populations purified from paediatic bronchoalveolar lavage samples. The cell types profiled are alveolar macrophages, granulocytes, lymphocytes and alveolar epithelial cells. Fluorescence-activated single cell sorting was used to purify the cell populations of interest using previously described methods (Shanthikumar, AJRCMB, 2020 ;63(2):152-159). Genome wide DNA methylation profiling was performed via the EPICArray. These data can be used for reference based deconvolution of cell proportion of bronchoalveolar lavage samples. DNA extracted from raw BAL (n=6) was also profiled using the EPICArray, allowing for validation of reference based deconvulution.
Project description:AJ mouse is susceptible to lung carcinogenesis from urethane treatment and is a good model for human adenocarcinoma. We completed a study using microarray analysis of bronchoalveolar lavage cells from control or urethane treated mice. A unique macrophage expression signature in the lung tumor microenvironment was able to correctly classify the lavage samples. Experiment Overall Design: RNA from bronchoalveolar lavage cells of age matched untreated AJ mice controls (C) or from urethane treated (T) AJ mice was prepared. Datasets were accurately classified using a unique macrophage gene expression signature derived from the tumor microenvironment.
Project description:We profiled DNA methylation, mRNA expression, and miRNA expression from bronchoalveolar lavage cells obtained from 64 sarcoidosis subjects and 16 healthy controls.
Project description:We profiled DNA methylation, mRNA expression, and miRNA expression from bronchoalveolar lavage cells obtained from 64 sarcoidosis subjects and 16 healthy controls.
Project description:We profiled DNA methylation, mRNA expression, and miRNA expression from bronchoalveolar lavage cells obtained from 64 sarcoidosis subjects and 16 healthy controls.