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Detection of revertant mosaicism in epidermolysis bullosa through Cas9-targeted long read sequencing

ABSTRACT: Detection of revertant mosaicism in epidermolysis bullosa through Cas9-targeted long read sequencing

PROVIDER: PRJNA771681 | ENA |

REPOSITORIES: ENA

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Detection of revertant mosaicism in epidermolysis bullosa through Cas9-targeted long read sequencing

Project description:Revertant mosaicism (RM) is a phenomenon in which inherited mutations are spontaneously corrected in somatic cells. RM occurs in some congenital skin diseases, although genetic validation of RM in clinically revertant skin has been challenging, especially when homologous recombination (HR) is responsible for RM. We introduced Nanopore Cas9-targeted sequencing (nCATS) for identifying HR in clinically revertant skin.

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