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Porphyromonas gingivalis W83:Transcriptome profiling and functional verification of flavodoxin

ABSTRACT: Porphyromonas gingivalis W83:Transcriptome profiling and functional verification of flavodoxin

PROVIDER: PRJNA773665 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16531845_1.fastq.gz	Fastqsanger.gz
	SRR16531845_2.fastq.gz	Fastqsanger.gz
	SRR16531846_1.fastq.gz	Fastqsanger.gz
	SRR16531846_2.fastq.gz	Fastqsanger.gz
	SRR16531847_1.fastq.gz	Fastqsanger.gz

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Porphyromonas gingivalis W83:Transcriptome profiling and functional verification of flavodoxin

Project description:The aim of the study was to identify the role of flavodoxin gene in the virulence of P. gingivalis W83.The mRNA profiles of P. gingivalis W83 and flavodoxin mutant strain were generated by deep sequencing, in triplicate, using Illumina sequencing platform. Differential expression analysis of two groups (Three biological replicates per group) was performed using the DESeq R package. In total, 376 genes met the DEGs criteria (194 upregulated genes and 182 down-regulated genes). The KEGG pathway analysis indicated the DEGs were significantly enriched in three KEGG pathways, comprising pathways associated with Peroxisome, Two-component system and Quorum sensing.Importantly, we found that the flavodoxin gene can regulate the type IX secretion system and oxidative stress system in P. gingivalis W83. The knowledge obtained will help us to deeply understand the role of flavodoxin gene in the P. gingivalis W83 genome.

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Expression profiling analyses of ECF sigma factor PG0162-PG1660 mutant of Porphyromonas gingivalis W83 [RNA-Seq]

Project description:The role of ECF sigma factors PG0162, PG01660 were involved in virulence regulationin Porphyromonas gingivalis was published Yuetan Dou, Devon Osbourne, Rachelle McKenzie, Hansel M Fletcher. (2010) Involvement of extracytoplasmic function sigma factors in virulence regulation in Porphyromonas gingivalis W83. FEMS Microbiology Letter, 312(1):24-32.

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Effect of deletion of trkA on gene expression of Porphyromonas gingivalis W83

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Expression profiling analyses of ECF sigma factor PG1660 mutant of Porphyromonas gingivalis W83

Project description:To study the expression profile of ECF sigma factor PG1660 mutant under anaerobic conditions and hydrogen peroxide stress conditions compared to the wild-type W83 by using DNA-microarray. The role of ECF sigma factor PG1660 involved in oxidative stress was published Yuetan Dou, Devon Osbourne, Rachelle McKenzie, Hansel M Fletcher. (2010) Involvement of extracytoplasmic function sigma factors in virulence regulation in Porphyromonas gingivalis W83. FEMS Microbiology Letter, 312(1):24-32.

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Comparison of Porphyromonas gingivalis W83 gene expression when grown with or without galactose

Project description:Porphyromonas gingivalis (P. gingivalis) W83 cultures in early exponential phase were supplemented with 0.1% galactose or vehicle control. The cultures were then permitted to grow anaerobically at 37°C for 3 hours, which corresponds to approximately one doubling of P. gingivalis. At this point, RNA extraction was performed using the cultures, and these samples were processed and submitted for RNA sequencing using an Illumina platform. Modest changes in gene expression were observed between cultures grown with or without galactose, and the majority of changes were associated with processes occurring at the cell membrane.

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Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity

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Porphyromonas gingivalis W83

Project description:Expression profiling analyses of ECF sigma factor PG1660 mutant of Porphyromonas gingivalis W83

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