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Programmed genomic instability regulates neural transdifferentiation of human brain microvascular pericytes [ChIP-Seq]

ABSTRACT: Programmed genomic instability regulates neural transdifferentiation of human brain microvascular pericytes [ChIP-Seq]

PROVIDER: PRJNA777272 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR16704790.fastq.gz	Fastqsanger.gz
	SRR16704791.fastq.gz	Fastqsanger.gz

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Programmed genomic instability regulates neural transdifferentiation of human brain microvascular pericytes [ChIP-Seq]

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Project description:The observation that human microvascular pericytes transdifferentiate into neurons provided an opportunity to explore the endogenous molecular basis for lineage reprogramming. Here, we show that abrupt destabilization of the higher-order chromatin topology that chaperones lineage memory of pericytes is driven by transient global transcriptional arrest. This leads within minutes to localized decompression of the repressed competing higher-order chromatin topology and expression of pro-neural genes. Transition to neural lineage is completed by probabilistic induction of R-loops in key myogenic loci upon re-initiation of RNA polymerase activity, leading to depletion of the myogenic transcriptome and emergence of the neurogenic transcriptome.

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Programmed genomic instability regulates neural transdifferentiation of human brain microvascular pericytes.

Project description:BackgroundTransdifferentiation describes transformation in vivo of specialized cells from one lineage into another. While there is extensive literature on forced induction of lineage reprogramming in vitro, endogenous mechanisms that govern transdifferentiation remain largely unknown. The observation that human microvascular pericytes transdifferentiate into neurons provided an opportunity to explore the endogenous molecular basis for lineage reprogramming.ResultsWe show that abrupt destabilization of the higher-order chromatin topology that chaperones lineage memory of pericytes is driven by transient global transcriptional arrest. This leads within minutes to localized decompression of the repressed competing higher-order chromatin topology and expression of pro-neural genes. Transition to neural lineage is completed by probabilistic induction of R-loops in key myogenic loci upon re-initiation of RNA polymerase activity, leading to depletion of the myogenic transcriptome and emergence of the neurogenic transcriptome.ConclusionsThese findings suggest that the global transcriptional landscape not only shapes the functional cellular identity of pericytes, but also stabilizes lineage memory by silencing the competing neural program within a repressed chromatin state.

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Genomic and transcriptomic profiles of human brain microvascular pericytes upon neural transdifferentiation

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