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Young transposable elements rewired gene regulatory networks in human and chimpanzee hippocampal intermediate progenitors

ABSTRACT: Young transposable elements rewired gene regulatory networks in human and chimpanzee hippocampal intermediate progenitors

PROVIDER: PRJNA782627 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR17008000_1.fastq.gz	Fastqsanger.gz
	SRR17008000_2.fastq.gz	Fastqsanger.gz
	SRR17008001_1.fastq.gz	Fastqsanger.gz
	SRR17008001_2.fastq.gz	Fastqsanger.gz

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Young transposable elements rewired gene regulatory networks in human and chimpanzee hippocampal intermediate progenitors

Project description:The hippocampus is associated with essential brain functions such as learning and memory. Human hippocampal volume is significantly greater than expected when compared to non-human apes, suggesting a recent expansion. Intermediate progenitors, which are able undergo multiple rounds of proliferative division before a final neurogenic division, may have played a role in the evolutionary hippocampal expansion. To investigate the evolution of gene regulatory networks underpinning hippocampal neurogenesis in apes, we leveraged the differentiation of human and chimpanzee induced Pluripotent Stem Cells into TBR2-positive hippocampal intermediate progenitors (hpIPCs). We find that the gene networks active in hpIPCs are significantly different between humans and chimpanzees, with ~2,500 genes differentially expressed. We demonstrate that species-specific transposon-derived enhancers contribute to these transcriptomic differences. Young transposons, predominantly Endogenous Retroviruses (ERV) and SINE-Vntr-Alus (SVA), were co-opted as enhancers in a species-specific manner. Human-specific SVAs provided substrates for thousands of novel TBR2 binding sites, and CRISPR-mediated repression of these SVAs attenuates the expression of ~25% of the genes that are upregulated in human intermediate progenitors relative to the same cell population in the chimpanzee

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