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Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence [scRNA-seq]

ABSTRACT: Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence [scRNA-seq]

PROVIDER: PRJNA792265 | ENA |

REPOSITORIES: ENA

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Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence

Project description:Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence

| PRJNA792267 | ENA

Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence [RNA-Seq]

Project description:Microglia serve critical remodeling roles that shape the developing nervous system, responding to the changing neural environment with phagocytosis or soluble factor secretion. Recent single-cell sequencing (scRNAseq) studies have revealed the context-dependent diversity in microglial properties and gene expression, but the cues promoting this diversity are not well defined. Here, we ask how interactions with apoptotic neurons shape microglial state, including lysosomal and lipid metabolism gene expression and dependence on Colony-stimulating factor 1 receptor (CSF1R) for survival. Using early postnatal mouse retina, a CNS region undergoing significant developmental remodeling, we performed scRNAseq on microglia from mice that are wild-type, lack neuronal apoptosis (Bax KO), or are treated with CSF1R inhibitor (PLX3397). We find that interactions with apoptotic neurons drives multiple microglial remodeling states, subsets of which are resistant to CSF1R inhibition. We find that TAM receptor Mer and complement receptor 3 are required for clearance of apoptotic neurons, but that Mer does not drive expression of remodeling genes. We show TAM receptor Axl is negligible for phagocytosis or remodeling gene expression but is consequential for microglial survival in the absence of CSF1R signaling. Thus, interactions with apoptotic neurons shift microglia towards distinct remodeling states and through Axl, alter microglial dependence on survival pathway, CSF1R.

2022-04-27 | GSE192601 | GEO

Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence [scRNA-seq]

2022-04-27 | GSE192600 | GEO

Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence [RNA-Seq]

Project description:Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence [RNA-Seq]

| PRJNA792269 | ENA

Neuronal apoptosis drives remodeling states of microglia and shifts in survival pathway dependence

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-04-27 | GSE192602 | GEO

Survival and PN Dependence in SBS Patients

Project description:Short bowel syndrome (SBS) is a rare condition resulting from the loss of portions of the small intestine, and can cause a spectrum of metabolic and physiologic disturbances.The objective of this study was to determine the longterm survival and parenteral nutrition dependence of adult patients with SBS.

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| PRJNA547655 | ENA

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Vitamin C Promotes Apoptosis in Breast Cancer Cells by Increasing TRAIL Expression

Project description:In this study we show that ascorbate (vitamin C) shifts the transcriptome of MDA-MB-231 cells and increases apoptosis.