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Metagenome

ABSTRACT: Glacier forefield soil metagenome

PROVIDER: PRJNA796041 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17635447_1.fastq.gz	Fastqsanger.gz
	SRR17635447_2.fastq.gz	Fastqsanger.gz
	SRR17635448_1.fastq.gz	Fastqsanger.gz
	SRR17635448_2.fastq.gz	Fastqsanger.gz
	SRR17635449_1.fastq.gz	Fastqsanger.gz

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Project description:Background: The soil environment is responsible for sustaining most terrestrial plant life on earth, yet we know surprisingly little about the important functions carried out by diverse microbial communities in soil. Soil microbes that inhabit the channels of decaying root systems, the detritusphere, are likely to be essential for plant growth and health, as these channels are the preferred locations of new root growth. Understanding the microbial metagenome of the detritusphere and how it responds to agricultural management such as crop rotations and soil tillage will be vital for improving global food production. Methods: The rhizosphere soils of wheat and chickpea growing under + and - decaying root were collected for metagenomics sequencing. A gene catalogue was established by de novo assembling metagenomic sequencing. Genes abundance was compared between bulk soil and rhizosphere soils under different treatments. Conclusions: The study describes the diversity and functional capacity of a high-quality soil microbial metagenome. The results demonstrate the contribution of the microbiome from decaying root in determining the metagenome of developing root systems, which is fundamental to plant growth, since roots preferentially inhabit previous root channels. Modifications in root microbial function through soil management, can ultimately govern plant health, productivity and food security.

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Quantifying the ecophysiology of growing microbes responding to warming along a productivity gradient of the Marr Ice Piedmont Glacier, West Antarctic Peninsula

Project description:Our overarching goal is to understand how microbes along a glacier forefield chronosequence and productivity gradient interact with their environment, how this influences carbon and nitrogen cycling, and how the microbes respond to temperature increases. Specifically, using the novel approach of quantitative SIP paired with metagenomic sequencing, we will calculate growth rates for targeted functional genes and metagenome assembled genomes, quantifying their ecophysiology, in situ. And when paired with gene expression using metatranscriptomic libraries and metabolite production, we will gain a clearer understanding of how microbes grow, how they cycle carbon and nitrogen and how their metabolic activity changes in response to warming. The work (proposal:https://doi.org/10.46936/10.25585/60008115) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.

2024-11-06 | MSV000096317 | MassIVE