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MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles

ABSTRACT: MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles

PROVIDER: PRJNA802039 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR17830221_1.fastq.gz	Fastqsanger.gz
	SRR17830221_2.fastq.gz	Fastqsanger.gz
	SRR17830222_1.fastq.gz	Fastqsanger.gz
	SRR17830222_2.fastq.gz	Fastqsanger.gz
	SRR17830223_1.fastq.gz	Fastqsanger.gz

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MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles [RNA-seq]

Project description:MafB (a bZIP transcription factor), ß-catenin (the ultimate target of the Wnt signal transduction pathway that acts as a transcriptional co-activator of LEF/TCF proteins), and WDR77 (a transcriptional co-activator of multiple hormone receptors) are important for breast cellular transformation. Unexpectedly, these proteins interact directly with each other, and they have similar genomic binding profiles. Furthermore, while some of these common target sites coincide with those bound by LEF/TCF, the majority are located just downstream of transcription initiation sites at a position near paused RNA polymerase (Pol II) and the +1 nucleosome. Occupancy levels of these factors at these promoter-proximal sites are strongly correlated with the level of paused Pol II and transcriptional activity.

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MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles [ChIP-seq]

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MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles [RNA-seq]

Project description:MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles [RNA-seq]

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MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles [ChIP-seq]

Project description:MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles [ChIP-seq]

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MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles.

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MafB, WDR77, and ß-catenin interact with each other and have similar genome association profiles

Project description:This SuperSeries is composed of the SubSeries listed below.

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Gene expression profiling of MafA, MafB, and MafA/MafB Mutants in E18.5 pancreas

Project description:MafA and MafB transcription factors have been shown to be key regulators of insulin and glucagon transcription. MafB is essential for alpha and beta cell differentiation, as MafB deficient mice produced fewer insulin+ and glucagon+ cells during development, with MafA expressed in remaining insulin+ cells. In contrast, beta cell development was reported to be normal in a total MafA knock out, although the animals developed beta cell dysfunction and diabetes as adults. However, we have found that MafB expression is elevated during development and retained in adult insulin+ cells after conditional removal of MafA in the pancreas. These studies will evaluate the broader significance of these insulin and glucagon regulators in alpha and beta cell development and function. Our efforts will focus on determining if the concerted actions of MafA and MafB factors are significant to beta cell formation, and we specifically plan to: Determine how alpha and beta cell differentiation is affected in MafA/MafB compound mutant mice during pancreas development. cDNA microarray studies (pancchip 6.0) with wild type, MafAKO, MafB-/-, and MafAKOMafB-/- mutant E18.5 pancreata will be performed to comprehensively identify genes controlled by MafA and MafB in developing alpha and beta cells.

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