Project description:We identified genes whose expression is upregulated in adult Drosophila m. brain 48h after a stab lesion by microarray analysis of RNA extracted from injured and intact wild-type brains (Oregon-R).
Project description:Study of genome-wide differential gene expression of adult Drosophila melanogaster prickle-prickle mutant brains compared to control animals.
Project description:Study of genome-wide differential gene expression of adult Drosophila melanogaster prickle-spiny legs mutant brains compared to control animals.
Project description:V5-TurboID was knock-in tagged in Drosophila caspase Drice which is expressed in adult brains. Flies were fed with 100 µM biotin for protein biotinylation in two biological replicates. Biotinylated protein-containing lysate extracted from adult brains was subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.
Project description:We report the transcriptional profiles from individual Drosophila melanogaster (whole bodies or dissected brains) to Entomophthora muscae at 24 time points following fungal exposure. In whole fruit fly bodies, a significant immune response is observed following exposure to the fungus. In brains, few differences are consistently observed between infected and uninfected animals.
Project description:We report the application of RNA-sequencing technology for high-throughput profiling of RNA abundance in Drosophila melanogaster brains. By obtaining RNA-sequencing reads, we generated quantitative transcriptome-wide measures in three nutritional states: sated, fasted, refed.
Project description:A spectral library was built for Drosophila melanogaster. The spectral library allows reproducible quantification for thousands of peptides per SWATH-MS analysis.
Proteins from Drosophila melanogaster embryo, adult flies were digested with trypsin using in-gel digestion and the peptides were fractionated by high-pH reverse phase chromatography. HRM peptides were spiked into the peptides mixture and each fraction was injected on a Sciex TripleTOF 6600 mass spectrometer fitted with microflow set-up.
The resulting .wiff files were analysed using MaxQuant and Spectronaut.
Project description:dG9a-depletion decreases the starvation resistance in the adult stage of Drosophila melanogaster. To determine which genes are regulated by dG9a under starvation stress, we examined mRNA levels by performing RNA-sequence analysis using 0 h and 12 h starved dG9a null mutant and wild type as a control.