Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

In vitro methylation studies in multiple human cell types

ABSTRACT: In vitro methylation studies in multiple human cell types

PROVIDER: PRJNA811634 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

In vitro methylation studies in multiple human cell types [HumanMethylation450 array]

Project description:In vitro methylation studies in multiple human cell types [HumanMethylation450 array]

| PRJNA811629 | ENA

In vitro methylation studies in multiple human cell types [HumanMethylation850 array]

Project description:In vitro methylation studies in multiple human cell types [HumanMethylation850 array]

| PRJNA811635 | ENA

In vitro methylation studies in multiple human cell types [HumanMethylation450 array]

Project description:An Infinium microarray (Illumina HumanMethylation450 BeadChip) was used to generate DNA methylation data from human tissue samples.

2022-03-03 | GSE197719 | GEO

In vitro methylation studies in multiple human cell types [HumanMethylation850 array]

Project description:An Infinium microarray (Illumina Infinium HumanMethylation850 BeadChip) was used to generate DNA methylation data from human tissue samples.

2022-03-03 | GSE197723 | GEO

In vitro methylation studies in human fibroblasts from progeria cases

Project description:In vitro methylation studies in human fibroblasts from progeria cases

| PRJNA823349 | ENA

In vitro methylation studies in multiple human cell types

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-03-03 | GSE197724 | GEO

Methylation studies in horses

Project description:Methylation studies in horses

| PRJNA731494 | ENA

Mapping of variable DNA methylation across multiple cell types defines a dynamic regulatory landscape of the human genome

Project description:DNA methylation is an important epigenetic modification involved in many biological processes and diseases. Many studies have mapped DNA methylation changes associated with embryogenesis, cell differentiation and cancer at a genome-wide scale. Our understanding of genome-wide DNA methylation changes in a developmental or disease-related context has been steadily growing. However, the investigation of which CpGs are variably methylated in different normal cell or tissue types is still limited. Here we present an in-depth analysis of 54 single-CpG-resolution DNA methylomes of normal human cell types by integrating high-throughput sequencing-based methylation data. We find that the percentage of unmethylated CpGs is relatively fixed regardless of cell type. However, which CpGs are unmethylated is cell-type specific. We categorize the 26 million human autosomal CpGs based on their methylation levels across multiple cell types to identify variably methylated CpGs. Among all the autosomal CpGs, 22.6% exhibit variable DNA methylation across cell types included in our study. These variably methylated CpGs form 66 thousand variably methylated regions (VMRs), encompassing 11% of the genome. By integrating a multitude of genomic data, we found that VMRs enrich for histone modifications indicative of enhancers, suggesting their role as regulatory elements marking cell type specificity. VMRs enrich for transcription factor binding sites in a tissue-dependent manner. Importantly, they enrich for GWAS variants, suggesting VMRs could potentially be implicated in disease and complex traits. Taken together, our results highlight the link among CpG methylation variation, genetic variation and disease risk in a tissue-specific manner for many human cell types. Processed data includes new Samples and 75 Samples from GSE16368 and 2 Samples from GSE51565.

2016-04-06 | E-GEOD-69894 | biostudies-arrayexpress

Methylation studies in plains zebras

Project description:Methylation studies in plains zebras

| PRJNA763600 | ENA

Mapping of variable DNA methylation across multiple cell types defines a dynamic regulatory landscape of the human genome

2016-04-06 | GSE69894 | GEO