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Human alveolar progenitors generate dual lineage bronchioalveolar organoids

ABSTRACT: Human alveolar progenitors generate dual lineage bronchioalveolar organoids

PROVIDER: PRJNA812919 | ENA |

REPOSITORIES: ENA

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Human alveolar progenitors generate dual lineage bronchioalveolar organoids

Project description:Mechanisms of epithelial renewal in the alveolar compartment remain incompletely understood. To this end, we aimed to characterize alveolar progenitors. Single-cell RNA-seq (scRNA-seq) analysis of the HTII-280 positive and HTII-280+ /EpCAM+populations from total 6 donors from periferal tissue was performed, and analysis revealed subclusters enriched for stem cell signature genes. We found that these alveolar progenitors in organoid culture in vitro show phenotypic lineage plasticity as they can yield alveolar or bronchial cell type progeny. The direction of the differentiation is dependent on the presence of the GSK-3β inhibitor, CHIR99021. By RNA-seq analysis of GSK-3 βknockdown organoids, we identify additional functional candidate target genes and pathways which contribute to alveolar differentiation independent of Wnt signaling

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