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Small RNA profile of 4 biofluids from healthy individuals B [EXR-KWANG1jo8Fqz-AN]

ABSTRACT: Small RNA profile of 4 biofluids from healthy individuals B [EXR-KWANG1jo8Fqz-AN]

PROVIDER: PRJNA820470 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18497600.fastq.gz	Fastqsanger.gz
	SRR18497601.fastq.gz	Fastqsanger.gz
	SRR18497602.fastq.gz	Fastqsanger.gz
	SRR18497603.fastq.gz	Fastqsanger.gz
	SRR18497604.fastq.gz	Fastqsanger.gz

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Small RNA profile of 4 biofluids from healthy individuals

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Small RNA profile of 4 biofluids from healthy individuals B [EXR-KWANG1jo8Fqz-AN]

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Small non-coding RNA profiling in human biofluids from healthy individuals

Project description:Small non-coding RNA profiling in human biofluids from healthy individuals

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Project description:The role of non-coding RNAs in different biological processes and diseases is continuously expanding. Next-generation sequencing together with the parallel improvement of bioinformatics analyses allows the accurate detection and quantification of an increasing number of RNA species. With the aim of exploring new potential biomarkers for disease classification, a clear overview of the expression levels of common/unique small RNA species among different biospecimens is necessary. However, except for miRNAs in plasma, there are no substantial indications about the pattern of expression of various small RNAs in multiple specimens among healthy humans. Overall, the data reported hereby provide an insight of the constitution of the human miRNome and other small non-coding RNAs in various specimens of healthy individuals. This dataset was submitted by the Extracellular RNA Atlas (http://exrna-atlas.org/exat/datasets/EXR-ANACC1S6lJ1C-AN), and the selected raw and processed data for this dataset corresponds to what is available in that resource. Submitter indicates: "The publication associated with the citation below refers to a slightly larger set of samples (includes cervical scrape samples) and contains an alternative analysis to the processed data files provided here."

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Project description:Small extracellular vesicles (sEV) are nano-sized (40-150 nm), membrane-encapsulated vesicles that are released by malignant or pathologic and non-pathologic cells into the extracellular space and function as intercellular signaling vectors through the horizontal transfer of biologic molecules, including microRNA (miRNA) and other small non-coding RNA (ncRNA), that can alter the phenotype of recipient cells. sEV are present in essentially all extracellular biofluids, including serum, urine and saliva, and offer a new avenue for discovery and development of novel biomarkers of various disease states and exposures. The objective of this study was to determine the similarities and differences between sEV ncRNA derived from saliva, serum and urine, as well as cell-free ncRNA from serum. Saliva, urine and serum were concomitantly collected from 4 healthy donors, and sEV were isolated from each respective biofluid, along with cfRNA from serum. sEVs were isolated from the respective biofluids via differential ultracentrifugation. Small RNA-sequencing was performed on each sample, and cluster analysis was performed based on ncRNA profiles. While some similarities existed in terms of sEV ncRNA cargo across biofluids, there are also notable differences in ncRNA class and ncRNA secretion, with each sEVs in each biofluid bearing a unique ncRNA profile. We conclude that sEV ncRNA cargo varies according to biofluid, so thus should be carefully selected and interpreted when designing translational or epidemiological studies.

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