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Effect of deletion of AZF1 on gene expression under the SEF1-deleted background in yeast Lachancea kluyveri

ABSTRACT: Effect of deletion of AZF1 on gene expression under the SEF1-deleted background in yeast Lachancea kluyveri

PROVIDER: PRJNA825085 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18694200.fastq.gz	Fastqsanger.gz
	SRR18694201.fastq.gz	Fastqsanger.gz
	SRR18694202.fastq.gz	Fastqsanger.gz
	SRR18694203.fastq.gz	Fastqsanger.gz
	SRR18694204.fastq.gz	Fastqsanger.gz

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Effect of deletion of AZF1 on gene expression under the SEF1-deleted background in yeast Lachancea kluyveri

Project description:The investigate the loss-of fucntion azf1 suppressive mutations to the sef1-deleted transcriptional netwrok in L. kluyveri, we analyzed the gene expression profiles in response to azf1 deletion under fermentative and respiratory conditions and foucsed on the compensatory effects on gene expression to sef1 deletion.

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Project description:To understand the Sef1-dependent gene expression in Lachancea kluyveri under both fermentative and respiratory conditions, we perfomred the genome-wide gene expression profiling for the log-phase cells of Lachancea kluyveri wild type and sef1 deletion mutant under both YPD and YPGly conditions.

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Sef1-VP16 dependent gene expression in Lachancea kluyveri

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Project description:Lachancea kluyveri (formerly Saccharomyces kluyveri); Growth on different nitrogen sources.

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Selenite treatment of a Lachancea kluyveri wild type strain

Project description:In the frame of a multispecies project, Lachancea kluyveri cells were treated with 5 mM sodium selenite. Cells were collected 10, 20, 30, 40, 50, 60, 70 and 80 minutes after the treatment and their transcriptomes were compared to those of mock-treated cultures. Four independent biological replicates were performed.

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Lachancea kluyveri (formerly Saccharomyces kluyveri); Growth on different nitrogen sources.

Project description:Transcriptional profiling of the yeast Lachancea kluyveri when grown on minimal media with different compounds added as the sole nitrogen source. Cells grown on uracil, uridine, dihydrouracil and ammonia were profiled using cells grown on proline as the reference. Five conditions, of one was used as reference. Each grown in 4 parallell biological replicates, which were extracted, labeled and hybridized individually, except for the reference where the replicates were pooled. 1 technical replicate. Please note that raw data files for the following samples are not available; Skluy_uracil_rep2, Skluy_dihydrouracil_rep3, Skluy_uridine_rep4

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