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ABSTRACT: Investigation of differential microRNAs in the exosomes of caerulein-induced AR42J pancreatic acinar cells

PROVIDER: PRJNA833775 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19020676.fastq.gz	Fastqsanger.gz
	SRR19020677.fastq.gz	Fastqsanger.gz
	SRR19020678.fastq.gz	Fastqsanger.gz
	SRR19020679.fastq.gz	Fastqsanger.gz
	SRR19020680.fastq.gz	Fastqsanger.gz

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Project description:Investigation of differential microRNAs in the exosomes of caerulein-induced AR42J pancreatic acinar cells

| PRJNA833775 | ENA

A modular analysis reveals that kinase-associated gene groups are involved in taurolithocholic acid 3-sulfate (TLC-S)-induced biological processes of AR42J cells

Project description:Background: Bile acids can act on pancreatic acinar cells and induce intracellular trypsinogen activation, but the underlying molecular mechanism remains unknown.The present study aimed to investigate taurolithocholic acid 3-sulfate (TLC-S)-induced alterations of gene groups in AR42J cells using a modular analysis.Methods: After the pancreatic acinar AR42J cells were stimulated by TLC-S for 20 min, gene chip technology was applied to identify genes that were differentially expressed. Subsequently, the regulatory sub-networks of genetic interactions among the differentially expressed genes were constructed using the protein–protein interaction (PPI) network. Finally, the CFinder software was utilized to generate genetic modules, which were then subjected to a functional enrichment analysis.Results: The gene chip assay demonstrated that the expression levels of 1124 genes were upregulated and that those of 498 genes were downregulated in the TLC-S

2014-12-09 | GSE63959 | GEO

Comprehensive analysis of microRNA signature of mouse pancreatic acini identifies over-expression of miR-21-3p in acute pancreatitis.

Project description:In the current study, we characterized the global miRNA expression profile in mouse pancreatic acinar cells during acute pancreatitis using next-generation RNA-Sequencing. We identified 330 known and 6 novel miRNAs being expressed in mouse pancreatic acinar cells. At basal state, miR-148a-3p, miR-375-3p, miR-217-5p, miR-216a-5p were among the most abundantly expressed whereas miR-24-5p and miR-421-3p were least abundant. Treatment of acinar cells with supra-maximal caerulein or bile acid induced numerous changes in miRNA expression levels. In particular, we observed significant upregulation of miR-21-3p in these experiments, which was further, confirmed using mouse models of acute pancreatitis. In summary, this is the first comprehensive analysis of miRNA expression in healthy mouse pancreatic acinar cells and how this expression signature changes in acute pancreatitis.

2016-10-25 | GSE81260 | GEO

Investigation of differential microRNAs in the exosomes of alcohol-induced H9c2 myocardial cells

Project description:Investigation of differential microRNAs in the exosomes of alcohol-induced H9c2 myocardial cells

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Gene expression profile of ADM, ductal and acinar cells in the setting of pancreatitis

Project description:In order to compare the gene expression profile of acinar-to-ductal metaplasia (ADM), ductal and acinar cells in the setting of pancreatitis, which were obtained by laser-capture microdissection (LMD) from frozen sections of wild type mice pancreata 2 days after caerulein administration, we performed microarray analysis. ADM has an intermediate property between ductal and acinar cells in the setting of pancreatitis, and Cxcr4 mRNA which is expressed in multipotent pancreatic progenitors, were up-regulated in ADM compared with ductal cells or acinar cells. Notably, in consistent with our immunostaining data, Dclk1 mRNA was highly expressed in ADM cells compared with ductal and acinar cells.

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Zonation of pancreatic acinar cells in diabetic mice

Project description:We sequenced AR42J cell exposed to CCK or insulin in different concentrations. This enable us to uncover the possible effect of the different hormones on acinar cells in pathlogy.

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Tuft cell formation reflects epithelial plasticity in pancreatic injury; implications for modeling human pancreatitis

Project description:We performed RNA-seq on pancreatic tuft cells and non-tuft epithelial cells in caerulein-induced pancreatitis

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Pancreatic acinar cell fate relies on system xc- to prevent ferroptosis during stress

Project description:Acinar cell dedifferentiation is one of the most notable features of acute and chronic pancreatitis. It can also be the initial step that facilitates pancreatic cancer development. In the present study, we further decipher the precise mechanism and regulation using murine experimental models. Our RNAseq analysis indicates that, early acinar cell dedifferentiation is accompanied by multiple pathways related to cell survival that are highly enriched, and where SLC7A11 (xCT) is transiently upregulated. xCT is the specific subunit of the cystine/glutamate antiporter system xC-. Acinar cells with depleted or reduced xCT function show an increase in ferroptosis relating to lipid peroxidation. Lower glutathione levels and more lipid ROS accumulation could be rescued by the antioxidant N-acetylcysteine or the ferroptosis inhibitor Ferrostatin-1. In caerulein-induced acute pancreatitis in mice, xCT also prevents lipid peroxidation in acinar cells. In conclusion, during stress, acinar cell fate seems to be poised for avoiding several forms of cell death. xCT specifically prevents acinar cell ferroptosis by fueling the glutathione pool and maintaining ROS balance. The data suggest that xCT offers a druggable tipping point to steer the acinar cell fate in stress conditions.

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2016-07-06 | E-MTAB-3921 | biostudies-arrayexpress

Insulin receptor signaling in pancreatic acinar cells contributes to KrasG12D-driven cancer initiation in the context of diet-induced obesity

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2023-10-19 | PXD033845 | Pride

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