Similar Datasets
Project description:Purpose: We conducted a reverse genetic approach in order to elucidate the role of H3K27me3 writers and erasers in the process of sex determination and expression in melon. Methods: We used TILLING to find loss-of function mutants of cmLHP1 proteins in melon. Selected alleles were phenotyped at the developmental and molecular levels. We generated the cmlhp1ab mutant, for which we observed a pleiotropic phenotype. For this double mutant, we integrated RNA-seq (leaves), ChIP-seq (leaves) and RT-PCR (flowers) for elucidating the impact of cmLHP1 loss of function at the molecular level. Results: cmlhp1ab displayed a pleiotropic phenotype which correlated with genome-wide changes of H3K27me3 (moore oftenly hypomethylation) and deregulation of genes involved in flower development and hormone responses.
2022-02-15 | GSE180385 | GEO
Project description:A human Pluripotent Stem Cell microglia model displays a neuronal-co-culture-specific expression profile and inflammatory response Walther Haenseler, Stephen N. Sansom, Julian Buchrieser, Sarah E. Newey, Craig S. Moore, Francesca J. Nicholls, Satyan Chintawar, Christian Schnell, Jack P. Antel, Nicholas D. Allen, M. Zameel Cader, Richard Wade-Martins, William S. James, Sally A. Cowley The aim of the experiment was to compare the gene expression profiles from human iPSC-derived embryonic macrophages (both precursors, mature, and cells cultured in 'microglia medium'), with iPSC-macrophages differentiated to microglia by co-culture with iPSC-derived cortical neurons. They were also compared to human blood-derived monocytes and to human primary fetal microglia.
2016-11-14 | GSE89795 | GEO
Project description:Purpose: We conducted a reverse genetic approach in order to elucidate the role of H3K27me3 writers and erasers in the process of sex determination and expression in melon. Methods: We used TILLING to find loss-of function mutants of cmLHP1 proteins in melon. Selected alleles were phenotyped at the developmental and molecular levels. We generated the cmlhp1ab mutant, for which we observed a pleiotropic phenotype. For this double mutant, we integrated RNA-seq (leaves), ChIP-seq (leaves) and RT-PCR (flowers) for elucidating the impact of cmLHP1 loss of function at the molecular level. Results: cmlhp1ab displayed a pleiotropic phenotype which correlated with genome-wide changes of H3K27me3 (moore oftenly hypomethylation) and deregulation of genes involved in flower development and hormone responses.
2022-02-15 | GSE180383 | GEO
Project description:These methylation data generated using EM-seq for all the NAM lines (as a part of the genome assembly project of NAM by the NAM Consortium Group). B73=project ID PRJEB32225/ERP114875; B73Ab10=project ID PRJEB35367/ERP118403; the rest of the NAMs=project ID PRJEB31061/ERP113571
2021-03-12 | E-MTAB-10088 | biostudies-arrayexpress
Project description:Plant material consisted of synthetic hexaploid wheat germplasm into the Opata background (Altar 84/ Aegilops squarrosa (TAUS)//Opata) . Plants were grown at a density of 9-11 individuals per 20cm x 10cm (diameter x height) plastic pot containing 1500g well-rinsed Turface MVP® medium (Profile Products LLC, Buffalo, IL), in controlled environment chambers at 23°C, 70% relative humidity, and 16h photoperiod with a photosynthetic photon flux (PPF) of 330±10 µmolem-2s-1 when measured at the top of the canopy at growth stage 22 to 24 in the Zadoks scale (Zadoks et al., 1974). Plants were watered daily until 21 days after seeding (DAS) by flooding trays with water for 5 minutes, then draining the trays. Drought stress was applied by water withholding, beginning on 21 DAS. Watering was withheld from plants belonging to drought treatment, while the control group received water above field capacity. Water content of the growth medium was gravimetrically monitored. Root tissues were collected from control and treated plants when the water content of the treatment group growth medium fell below the permanent wilting point. Three biological replicates were conducted in three separate time-span courses. Total RNA was isolated using a LiCl3 precipitation method following Moore et al., (2005). Dye swap desin microarray analyses of well-watered and water-stressed root tissues were conducted across three biological replicates totaling six hybridizations.
2006-12-01 | E-MEXP-831 | biostudies-arrayexpress
Project description:This dataset is part of ERC funded HumEn project (http://www.hum-en.eu/). This dataset relates to standardization of genome wide DNase 1 hypersensitivity methods in the differentiation system developed in HumEn project.
2022-11-19 | GSE108623 | GEO