Project description:The coordination of chloroplast and nuclear genome status are critical for plant cell function, but the mechanism remain largely unclear. In this study, we report that Arabidopsis thaliana CHLOROPLAST AND NUCLEUS DUAL-LOCALIZED PROTEIN 1 (CND1) maintains genome stability in both the chloroplast and the nucleus.
Project description:Microarray analysis of two nonphotosynthetic nuclear mutants (mcd1-1, mcd1-2) that affect chloroplast RNA stability to identify affected organellar genes (chloroplast and mitochondrial). Further, the photosynthetic suppressor (mcd2-1) that suppresses mcd1-2 was included to determine which genes respond directly to the RNA stabilty phenotype and which respond to the nonphotosynthetic phenotype.
Project description:Microarray analysis of two nonphotosynthetic nuclear mutants (mcd1-1, mcd1-2) that affect chloroplast RNA stability to identify affected organellar genes (chloroplast and mitochondrial). Further, the photosynthetic suppressor (mcd2-1) that suppresses mcd1-2 was included to determine which genes respond directly to the RNA stabilty phenotype and which respond to the nonphotosynthetic phenotype. Keywords: repeat sample
Project description:The coordination of chloroplast and nuclear genome status are critical for plant cell function, but the mechanism remain largely unclear. In this study, we report that Arabidopsis thaliana CHLOROPLAST AND NUCLEUS DUAL-LOCALIZED PROTEIN 1 (CND1) maintains genome stability in both the chloroplast and the nucleus.
2022-12-13 | GSE220490 | GEO
Project description:chloroplast and mitochondrial genomes
Project description:The regulator for chloroplast biogenesis (rcb) mutant was identified as a mutant defective in phytochrome-mediated chloroplast biogenesis. The rcb mutant has long hypocotyl and albino phenotypes. RCB initiates chloroplast biogenesis in the nucleus by promoting the degradation of the master repressors for chloroplast biogenesis, the PIFs (Phytochrome Interacting Factors). To understand how RCB regulates the expression of PIF-regulated genes, we performed genome-wide expression analysis of RCB-dependent genes using a rcb-10 null allele.
Project description:This dataset provides the transcriptome of the central cell of the female gametophyte from Arabidopsis thaliana. We sequenced two biological replicates. Each replicate started with a pool of 450 central cells collected using laser-assisted microdissection (LAM). Libraries were prepared as described in DOI:10.1038/NMETH.1315. Each library was sequenced on one eight of a slide on the AB SOLiD platform (version 3). IMPORTANT: In the alignment files (.bam), we named the mitochondrial and the chloroplastidial chromosomes ChrM and ChrC. This is different from the "chloroplast" and "mitochondrium" names in the whole genome fasta file available on TAIR.
Project description:Deep sequencing provided evidence that a novel subset of small RNAs were derived from the chloroplast genome of Chinese cabbage (Brassica rapa) and Arabidopsis (Ler). The chloroplast small RNAs (csRNAs) include those derived from mRNA, rRNA, tRNA and intergenic RNA. The rRNA-derived csRNA were preferentially located at the 3â-ends of the rRNAs, while the tRNA-derived csRNAs were mainly located at 5â-termini of the tRNAs. After heat treatment, the abundance of csRNAs decreased in chinese cabbage seedlings, except those of 24 nt in length. The novel heat-responsive csRNAs and their locations in the chloroplast were verified by Northern blotting. The regulation of some csRNAs to the putative target genes were identified by real-time PCR. Our results indicated that high temperature regulated the production of some csRNAs, which may have potential roles in transcriptional or post-transcriptional regulation, and affected putative target genes expression in chloroplast.
Project description:Changes ins organellar gene expression trigger retrograde signalling. Prolyl-tRNA synthetase (PRORS1) is located in chloroplasts and mitochondria. Thus, prors1-2 mutants are impaired in chloroplast and mitochondrial gene expression. The effects of the prors1-2 mutation on the global transcriptome were investigated with microarray analyses.
