Saccharomyces cerevisiae
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA91985 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:BMA64 rrp6 delta::HIS3 vs BMA64 WT (Mat a), grown at 30°C to OD 0.6 in YPD. BMA64 rrp6 delta::URA3, trf4 delta::KAN vs BMA64 WT, grown at 30°C to OD<0.6 in YPD.
2008-06-12 | E-GEOD-2579 | biostudies-arrayexpress
Project description:BMA64 rrp6 delta::HIS3 vs BMA64 WT (Mat a), grown at 30°C to OD 0.6 in YPD. BMA64 rrp6 delta::URA3, trf4 delta::KAN vs BMA64 WT, grown at 30°C to OD<0.6 in YPD. Keywords: other
2005-04-28 | GSE2579 | GEO
Project description:Wild-type yeast vs delta esl1/ delta esl2 mutant strain
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Project description:Mapping Nucleosome positions in WT and delta isw2 cells
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Project description:Comparsion of multiple BC k56-2 muants to assess protoeme chnages. seven strain comparsion between WT vs delta pglL vs delta 1086 vs delta 2974 vs delta CepI vs delta CepR vs delta pglL complemented AmrAB::S7-pglL-his. LFQ based quantification
2019-11-01 | PXD014581 | Pride
Project description:Characterisation of the effect of glycosylation disruption within Burkholderia cenocepacia k56-2. Five strain comparsion (WT vs delta pglL vs delta OGC vs Delta pglL OGC vs Delta pglL complemented S7-pglL-his)
2019-11-01 | PXD014429 | Pride
Project description:Purpose: The exosome plays major roles in RNA processing and surveillance but the in vivo target range and substrate acquisition mechanisms remain unclear. We applied an in vivo cross-linking technique coupled with deep sequencing (CRAC) that captures transcriptome-wide interactions between individual yeast exosome subunits and their targets in a living cell. Methods: We apply CRAC to HTP-tagged proteins (HTP: His6 - TEV cleavage site - two copies of the z-domain of Protein A): Two nucleases (Rrp44, Rrp6) and two structural subunits (Rrp41, Csl4) of the yeast exosome. At least two independent experiments were performed in each case and analyzed separately. We performed CRAC on wild-type (WT) Rrp44 and two catalytic mutants, rrp44-endo (D91N, E120Q, D171N, D198N) and rrp44-exo (D551N). We further developed CRAC using cleavable proteins (split-CRAC) to compare endonuclease and exonuclease targets of Rrp44. Plasmids designed for split-CRAC contain a PreScission protease cleavage site (PP) inserted between aa 241 and 242 in the RRP44 ORF to allow in vitro cleavage of purified protein, and a His6 tag to select the respective cleaved fragment. Results: Analysis of wild-type Rrp44 and catalytic mutants showed that both the CUT and SUT classes of noncoding RNA, snoRNAs and, most prominently, pre-tRNAs and other Pol III transcripts are targeted for oligoadenylation and exosome degradation. Unspliced pre-mRNAs were also identified as targets for Rrp44 and Rrp6. CRAC performed using cleavable proteins (split-CRAC) revealed that Rrp44 endonuclease and exonuclease activities cooperate on most substrates. Mapping oligoadenylated reads suggests that the endonuclease activity may release stalled exosome substrates. Rrp6 was preferentially associated with structured targets, which frequently did not associate with the core exosome. This indicates that substrates can follow multiple pathways to the nucleases. Conclusion: Our study represents the first transcriptome-wide map of substrates for the yeast exosome nuclease complex.
2012-09-25 | GSE40046 | GEO
Project description:Characterisation of the effect of multiple pglL muants and complement within Burkholderia cenocepacia k56-2 to confirm glycosylation phenotype. Six strain comparsion (WT vs delta pglL 1 vs delta pglL 2 vs delta pglL 1 complement native pglL vs delta pglL 1 complement S7-pglL-his vs delta pglL 2 complement native pglL
2019-11-01 | PXD014516 | Pride