Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA93903 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Mice were injected intravenously with 1st generation or 2nd generation Ad vectors (each bearing a LacZ transgene) or mock infected (controls) to completely transduce the liver. At different time points post-injection (6 hours, 3 days, and 3 weeks (21 days)), the liver was harvested and the overall transcriptome response to viral infection was assayed to measure the cellular immune response. Keywords: Immune Response
2005-12-15 | GSE3739 | GEO
Project description:Human immunodeficiency viruses type 1 and 2 (HIV-1 and 2) are known to depend on cellular host machinery for their replication and survival. While most of the studies on cellular proteomic and transcriptomic changes focused on the late-phase of the infection, studies of those changes in the early time-points; especially in the case of HIV-2 infection, are widely lacking. Using 2nd generation HIV-1 and 2 VSV-G pseudotyped lentiviral vectors, we transduced HEK-293T cells, and carried out transcriptomic profiling and proteomic analysis of harvested cells at 2h time point, which is representative of the immediate early-phase events in the infection cycle.
2021-11-03 | PXD024243 | Pride
Project description:The present work focuses on global gene expression of peripheral blood mononuclear cells in rhesus monkey infants with HFMD induced by CA16 infection at different time points. Genome-wide expression and analysis were performed by using Agilent whole genome microarrays and established bioinformatics tools. 931 signiM-oM-,M-^Acantly differentially expressed genes associated with 5 gene ontology categories, including cell communication, cell cycle, immune system process, regulation of transcription and metabolic process were identified. Subsequently, by mapping genes related to immune system process on KEGG pathways, the predominance of inflammation mediate by chemokine and cytokine signaling pathway and interleukin signaling pathway emerged. Ultimately, co-expression genes and their networks were analysised. Coxsackievirus A16 infection induced gene expression in rhesus monkey peripheral blood mononuclear cells was measured at 7-1st dpi, 3-2nd dpi, 7-2nd dpi, 14-2nd dpi. Three samples from two experiment donors and one control donor were performed at each time. To exclude the individual difference, mix the two experiment samples at each time, and mix the two control samples.
2015-03-10 | E-GEOD-66757 | biostudies-arrayexpress
Project description:Overall, the study aims at obtaining a comprehensive picture of the African malaria mosquito, Anopheles gambiae, transcriptome using high-coverage RNA-seq of sexed whole-insect samples collected at different developmental time points. This experiment focuses on transcriptomes of 1st, 2nd, 3rd and 4th instar male and female larvae, and 2 day old male and female adults.
2015-04-01 | E-MTAB-2527 | biostudies-arrayexpress
Project description:The innate immune response to 1st generation adenoviral vectors was ascertained through high level infection of primary cells from a inbred mouse strain, replete with an intact and functioning TLR system. Experiment Overall Design: Quiescent murine C57/Bl6 fibroblasts were Ad (E1-E3-LacZ at MOI=300) or Mock infected. Sixteen hrs post-infection, RNA was harvested, transcribe to cDNA and bitotin-labeled, and hybridized to Mu11k A and B arrays.
2007-12-04 | E-GEOD-3172 | biostudies-arrayexpress
Project description:Human immunodeficiency viruses type 1 and 2 (HIV-1 and 2) are known to depend on the cellular machinery for their replication and survival. While most of the studies on cellular proteomic and transcriptomic changes focused on the late-phase of the infection, studies of those changes in early time-points; especially in the case of HIV-2, are widely lacking. Using 2nd generation HIV-1 and 2 VSV-G pseudotyped lentiviral vectors, we transduced HEK-293T cells, and carried out transcriptomic profiling and proteomic analysis at 0 and 2h time points. Significant variations were observed in gene expression profile between HIV-1 and HIV-2 transduced samples. Thrombospondin 1 (THBS1), collagens (COL1A2, COL3A1) and eukaryotic translation factors (EIF3CL), in addition to various genes coding for long-non-coding RNA (lncRNA) were significantly upregulated at 2h time-point after HIV-2 transduction, compared to HIV-1. Label-free quantification mass spectrometry indicated that 7 proteins involved in RNA binding, mRNA transport, and chaperoning were significantly downregulated. Identification of cellular protein targets of HIV, and their effect on the cellular transcriptome will undoubtedly shed more light on their complex life cycle, and may be utilized against the infection, furthermore, characterizing the early-phase of HIV-2 infection may aid in the understanding of its pathomechanism, and long incubation period.
2021-02-20 | GSE167098 | GEO
Project description:Human mononuclear cells were cultured in 2 phases. In the 1st phase the culture medium contained cyclosporine A the 2nd phase contained SCF and erythropoietin. Cells were collected at 3 stages of differentiation; on day 6, 10, 12 and represented early erythroblasts, medium stage and normoblasts. Keywords: time course
2009-03-31 | GSE12407 | GEO
Project description:Question Addressed: How does Simian immunodeficiency virus (SIV) infection alter gene expression in memory CD4 T cell subsets very early during the 1st 10 days after infection? Memory CD4 T cells were sorted from rhesus macaque peripheral blood samples before infection and then at 4 and 10 days post SIV infection. RNA was recovered from the sorted memory CD4 T cells samples. RNA from the time point prior to SIV infection from each individual animal was used as the reference for the post SIV infection time points for that same animal. Thus, for each data set, the 4 and 10 day time points are being directly compared to the pre-infection data from the same animal. RNA samples as indicated above were used for reverse transcription reactions that directly incorporate Cy5 and Cy3 labeled nucleotides into the cDNA. Time: Time after infection with SIV
2019-09-23 | GSE38917 | GEO
Project description:Natural killer (NK) cells are innate lymphocytes that possess features of adaptive immunity such as clonal expansion and generation of long-lived memory. Here we look at transcriptional profiles of NK cells throughout several time points during MCMV infection, ex-vivo cytokine stimulation, and/or deficiency of key transcription factors such as STAT4, STAT1, and Runx1. In addition, we profile parallel time points of MCMV-specific CD8 T cells during infection and memory formation.
2017-12-20 | GSE106138 | GEO
Project description:While recent studies of immunity have uncovered many aspects of the innate immune system, there has been precious little investigation of the cellular response to viruses in vivo. To this end, we exploited high titer adenoviral (Ad) vectors to investigate the cellular response to non-enveloped viral infection in vivo. Our results indicate a potent cellular transcriptome response early after infection, with global assessments revealing significant dysregulation in ~15% of measured transcripts derived from Ad infected tissue. Transcriptome analysis revealed a complex interplay between the innate and adaptive responses, with suppression of metabolism and mitochondrial genes akin to those observed when mice are challenged with LPS. But despite this common response profile, there were many unique aspects of the Ad dependent trancriptome response, including upregulation of several RNA regulatory mechanisms and apoptosis-related pathways, accompanied by suppression of lysosome, endocytic, Wnt, and Calcium signaling pathways. Investigations into the TLR pathway using MyD88KO mice revealed that Ad induction of the TLR, MAPK, and cytokine receptor genes are significantly dependent on MyD88, as well as five other functional gene modules (mitochondrial, RNA regulation, cell cycle and growth, extracellular, and immune response genes). Absence of MyD88 also resulted in a greatly diminished Th1 response and acute phase response at later time points, confirming the important role MyD88 plays as an anti-adenoviral immunity amplifier and regulator in vivo. However, continued activation of immune response genes in Ad infected MyD88KO mice indicates that MyD88 is not the only component of the Ad ‘sensing mechanism’ in vivo. Keywords: Infectious response, pathogen response
2006-04-04 | GSE4339 | GEO