Lactiplantibacillus plantarum WCFS1
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ABSTRACT:
PROVIDER: PRJNA97299 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with oleuropein. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile shows that Lactobacillus plantarum WCFS1 adapts its metabolic capacity to acquire certain carbohydrates and repress the expression of genes involved in fatty acid biosyntheis. The transcriptomic datasets also revealed the downregulation of genes related to the biosynthesis of capsular polysaccharides and genes coding for ABC-type transporters. In addition, induction of oligopeptide permeases is also part of the response of Lactobacillus plantarum WCFS1 to oleuropein.
2019-10-21 | GSE90719 | GEO
Project description:Transcriptome profiles of control Lactobacillus plantarum WCFS1 cells were compared with 8% ethanol adapted cells and with 10 min or 30 min 8% ethanol shocked cells.
2010-01-01 | GSE17847 | GEO
Project description:In order to understand LBG derived galacto-manno-oligosaccharides utilization by a probiotic bacterium, Lactobacillus plantarum WCFS1, we have grown Lactobacillus plantarum WCFS1 (in duplicates) till mid log phase (OD600nm ~0.5, 10 h) in carbon free MRS (de Man, Rogosa Sharpe ) media containing either galacto-manno-oligosaccharides, mannose, glucose or galactose (1% w/v) as the sole carbon source.
2019-07-30 | GSE117591 | GEO
Project description:Ethanol stress response in Lactobacillus plantarum WCFS1
| PRJNA117907 | ENA
Project description:Lactobacillus plantarum WCFS1 was grown under anaerobic carbon-limited conditions in a chemostat with complete biomass retention (retentostat). In this cultivation system, the biomass concentration progressively increases while the dilution rate is kept constant, resulting in decreased specific susbtrate availibility, and hence, a progressive decrease in the specific growth rate. During the progressive transition from growth to virtually no growth, the global changes occurring at the level of metabolism and gene expression were studied using a genome-scale metabolic model and DNA microarrays.
2010-01-01 | GSE18340 | GEO
Project description:Lactobacillus plantarum was grown anaerobically on 4 different sugars (Mannose Lactose Fructose and Sucrose) to OD600 = 1.0. Samples were compared with a similar grown culture on glucose. An independnet biological duplicate of tht experimnet was performed (samples 1 and 2). L. plantarum WCFS1 grown in glucose, mannose, fructose, and sucrose
2010-09-15 | E-GEOD-24098 | biostudies-arrayexpress
Project description:Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals the induction of genes related to defense mechanisms against oxidative challenge. This response include genes related to inactivation of toxic oxygen radicals, probably derived from the autooxidation of hydroxytyrosol and genes involved in repairing the damage of oxygen radicals on proteins, including those involved in the metabolism of sulphur amino acids. Genes involved in peptidoglycan turnover, strenghthening of the cell wall and genes coding for osmoprotectants transporters, were downregulated. In addition, genes coding for elements typically involved in the stringent response, including ribosomal proteins, enzymes involved in the metabolism of the ppGpp alarmone, nitrogen metabolism, cell division and general stress proteins, were differentially regulated by hydroxytyrosol.
2020-06-17 | GSE90714 | GEO
Project description:trxB1 overexpression response to hydrogen peroxide stress in Lactobacillus plantarum WCFS1
| PRJNA101359 | ENA
Project description:Short-chain fructooligosaccharides (scFOS) and other prebiotics are used to selectively stimulate the growth and activity of lactobacilli and bifidobacteria in the colon. However, there is little information on the mechanisms whereby prebiotics exert their specific effects upon such microorganisms. To study the genomic basis of scFOS metabolism in Lactobacillus plantarum WCFS1, two-colour microarrays were used to screen for differentially expressed genes when grown on scFOS as compared to glucose (not a prebiotic). A significant up-regulation (8 to 60-fold) was observed with a set of only five genes located in a single locus and predicted to encode a sucrose phosphoenolpyruvate transport system (PTS), a â-fructofuranosidase, a fructokinase, an á-glucosidase and a sucrose operon repressor. Several other genes were slightly overexpressed, including pyruvate dehydrogenase. For the latter, no detectable activity in L. plantarum under various growth conditions, has been previously reported. A mannose-PTS likely to encode glucose uptake was 50-fold down-regulated as well as, to a lower extent, other PTS. Chemical analysis of the different moieties of scFOS that were depleted in the growth medium revealed that the trisaccharide 1-kestose present in scFOS was preferentially utilized, in comparison with the tetrasaccharide nystose and the pentasaccharide fructofuranosylnystose. The main end-products of scFOS fermentation were lactate and acetate. This is the first example in lactobacilli of the association of a sucrose PTS and a â-fructofuranosidase for scFOS degradation. Keywords: comparative transcriptomics, carbohydrate metabolism
2007-02-13 | GSE5882 | GEO
Project description:Genome-wide prediction and validation of σ70 promoters in Lactobacillus plantarum WCFS1
| PRJNA157151 | ENA