Dataset Information

Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates

ABSTRACT: Background. Many microbial hosts used for the rapid discovery and development of metabolic pathways can have sensitivities to final products and process reagents. Isopentenol, a biogasoline candidate, has an established heterologous gene pathway but is toxic to many microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. We explored the use of Corynebacterium glutamicum as an alternative host to address these constraints. Results. We found C. glutamicum ATCC 13032 to be tolerant to both the final product, isopentenol, as well to three classes ionic liquids. A heterologous mevalonate-based isopentenol pathway was engineered in C. glutamicum. Isopentenol titers were improved via three routes: media optimization; substitution of an NADH-dependent HmgR homolog from Silicibacter pomeroyi; and development of a C. glutamicum ∆poxB ∆ldhA host chassis.

ORGANISM(S): Corynebacterium Glutamicum Atcc 13032

SUBMITTER: Chris Petzold

PROVIDER: PXD012293 | panorama | Wed Apr 03 00:00:00 BST 2019

REPOSITORIES: PanoramaPublic

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Engineering <i>Corynebacterium glutamicum</i> to produce the biogasoline isopentenol from plant biomass hydrolysates.

Sasaki Yusuke Y Eng Thomas T Herbert Robin A RA Trinh Jessica J Chen Yan Y Rodriguez Alberto A Gladden John J Simmons Blake A BA Petzold Christopher J CJ Mukhopadhyay Aindrila A

Biotechnology for biofuels 20190227

<h4>Background</h4>Many microbes used for the rapid discovery and development of metabolic pathways have sensitivities to final products and process reagents. Isopentenol (3-methyl-3-buten-1-ol), a biogasoline candidate, has an established heterologous gene pathway but is toxic to several microbial hosts. Reagents used in the pretreatment of plant biomass, such as ionic liquids, also inhibit growth of many host strains. We explored the use of <i>Corynebacterium glutamicum</i> as an alternative h ...[more]

PMID: 30858878

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Dataset Information

Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates

Publications

Engineering <i>Corynebacterium glutamicum</i> to produce the biogasoline isopentenol from plant biomass hydrolysates.

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