Proteomics

Dataset Information

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Absolute Quantification of Schistosoma mansoni Esophageal Gland Products


ABSTRACT: Schistosomes are blood-dwelling helminth parasites causing a debilitating disease in the tropics. Major challenges to control the disease persist and vaccines would provide an additional tool, but their development has been problematic. Rhesus macaques can self-cure following schistosome infection, generating antibodies that target schistosome proteins from the tegument, gut and esophagus, the last of which is the least investigated. We developed a dissection technique that permitted comparative proteomics of the schistosome esophagus and gut for detection of secreted antigens. Shotgun proteome analysis of the male schistosomes esophagus identified 13 proteins encoded by microexon genes (MEG), eleven of which were uniquely located in the esophageal glands. Based on this and transcriptome information, a QconCAT was designed for absolute quantification of selected targets. MEGs 12, 4.2, 4.1 and Venom allergen-like protein 7 were the mostly abundant, spanning over 245-6 million copies per cell, while aspartyl protease, palmitoyl thioesterase and a galactosyl transferase were present at <1 million cpc. Antigenic variation by alternative splicing of MEG proteins was confirmed together with a specialised machinery for protein glycosylation in the esophagus. Moreover, some gastrodermal secretions were highly enriched in the gut, while others were more uniformly distributed throughout the parasite, potentially indicating lysosomal activity. Collectively, our findings provide a more rational, better-oriented selection of schistosome vaccine candidates in the context of a proven model of protective immunity.

ORGANISM(S): Schistosoma Mansoni

SUBMITTER: Leandro Neves  

PROVIDER: PXD014899 | panorama | Fri Nov 22 00:00:00 GMT 2019

REPOSITORIES: PanoramaPublic

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Publications

Quantitative Proteomics of Enriched Esophageal and Gut Tissues from the Human Blood Fluke <i>Schistosoma mansoni</i> Pinpoints Secreted Proteins for Vaccine Development.

Neves Leandro X LX   Wilson R Alan RA   Brownridge Philip P   Harman Victoria M VM   Holman Stephen W SW   Beynon Robert J RJ   Eyers Claire E CE   DeMarco Ricardo R   Castro-Borges William W  

Journal of proteome research 20191202 1


Schistosomes are blood-dwelling helminth parasites that cause schistosomiasis, a debilitating disease resulting in inflammation and, in extreme cases, multiple organ damage. Major challenges to control the transmission persist, and the discovery of protective antigens remains of critical importance for vaccine development. Rhesus macaques can self-cure following schistosome infection, generating antibodies that target proteins from the tegument, gut, and esophagus, the last of which is the least  ...[more]

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