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Phage Phix174 - Wildtype and engineered proteome

ABSTRACT: Sequence overlap between two genes is common across all genomes, with viruses having particularly high proportions of these gene overlaps. The natural biological function and effects on fitness of gene overlaps are not fully understood and their effects on gene cluster and genome-level refactoring are unknown.The model bacteriophage φX174 genome displays complex sequence architecture in which ~26% of nucleotides are involved in encoding more than one gene. In this study we use an engineered φX174 phage containing a genome with all gene overlaps removed. Here we have temporally measured the proteome of a synthetically engineered and wild-type φX174 during infection. We find that almost half of all phage proteins (5/11) have abnormal expression profiles after genome modularisation.

ORGANISM(S): Escherichia Virus Phix174 Synthetic Enterobacteria Phage Phix174.1f

SUBMITTER: Bradley Wright

PROVIDER: PXD019681 | panorama | Tue Jun 15 00:00:00 BST 2021

REPOSITORIES: PanoramaPublic

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Genome Modularization Reveals Overlapped Gene Topology Is Necessary for Efficient Viral Reproduction.

Wright Bradley W BW Ruan Juanfang J Molloy Mark P MP Jaschke Paul R PR

ACS synthetic biology 20201012 11

Sequence overlap between two genes is common across all genomes, with viruses having high proportions of these gene overlaps. Genome modularization and refactoring is the process of disrupting natural gene overlaps to separate coding sequences to enable their individual manipulation. The biological function and fitness effects of gene overlaps are not fully understood, and their effects on gene cluster and genome-level refactoring are unknown. The bacteriophage φX174 genome has ∼26% of nucleotid ...[more]

PMID: 33044064

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