Dataset Information

Targeted mass spectrometry enables multiplexed quantification of immunomodulatory proteins in clinical biospecimens

ABSTRACT: Immunotherapies are revolutionizing cancer care, producing durable responses and potentially cures in a subset of patients. However, response rates are low for most tumors, grade 3/4 toxicities are not uncommon, and our current understanding of tumor immunobiology is incomplete. While hundreds of immunomodulatory proteins in the tumor microenvironment shape the anti-tumor response, few of them can be reliably quantified. To address this need, we developed a multiplex panel of targeted proteomic assays to 52 peptides representing 46 proteins using peptide immunoaffinity enrichment coupled to multiple reaction monitoring-mass spectrometry. We validated the assays in tissue and plasma matrices, where performance figures of merit showed over 3 orders of dynamic range and median inter-day CVs of 5.2% (tissue) and 21% (plasma). A feasibility study in clinical biospecimens showed detection of 48/52 peptides in frozen tissue and 38/52 peptides in plasma. The assays are publicly available as a resource for the research community.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jeff Whiteaker

PROVIDER: PXD029167 | panorama | Mon Feb 28 00:00:00 GMT 2022

REPOSITORIES: PanoramaPublic

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Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.

Whiteaker Jeffrey R JR Lundeen Rachel A RA Zhao Lei L Schoenherr Regine M RM Burian Aura A Huang Dongqing D Voytovich Ulianna U Wang Tao T Kennedy Jacob J JJ Ivey Richard G RG Lin Chenwei C Murillo Oscar D OD Lorentzen Travis D TD Thiagarajan Mathangi M Colantonio Simona S Caceres Tessa W TW Roberts Rhonda R RR Knotts Joseph G JG Reading Joshua J JJ Kaczmarczyk Jan A JA Richardson Christopher W CW Garcia-Buntley Sandra S SS Bocik William W Hewitt Stephen M SM Murray Karen E KE Do Nhan N Brophy Mary M Wilz Stephen W SW Yu Hongbo H Ajjarapu Samuel S Boja Emily E Hiltke Tara T Rodriguez Henry H Paulovich Amanda G AG

Frontiers in immunology 20211111

Immunotherapies are revolutionizing cancer care, producing durable responses and potentially cures in a subset of patients. However, response rates are low for most tumors, grade 3/4 toxicities are not uncommon, and our current understanding of tumor immunobiology is incomplete. While hundreds of immunomodulatory proteins in the tumor microenvironment shape the anti-tumor response, few of them can be reliably quantified. To address this need, we developed a multiplex panel of targeted proteomic ...[more]

PMID: 34858420

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Dataset Information

Targeted mass spectrometry enables multiplexed quantification of immunomodulatory proteins in clinical biospecimens

Publications

Targeted Mass Spectrometry Enables Multiplexed Quantification of Immunomodulatory Proteins in Clinical Biospecimens.

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