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Targeted Proteomics of Lung Squamous Cell Carcinomas

ABSTRACT: To address the complexity of cancer, liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM) panels provide the capability to quantify clinical biomarkers and emerging protein markers to establish the context of tumor phenotypes that provide highly relevant supporting information. The resulting targeted proteomics data will empower translational researchers to move protein biomarker panels through the process from discovery to clinical use. Here, targeted proteomics panel measurements provide pathway-level evaluations of key biological drivers (e.g., EGFR signaling), phenotypes (e.g., EMT) and the ability to quantify specific drug targets within the context of a sample cohort of lung squamous cell carcinomas.

ORGANISM(S): Homo Sapiens

SUBMITTER: John Koomen

PROVIDER: PXD040680 | panorama | Sun Jul 09 00:00:00 BST 2023

REPOSITORIES: PanoramaPublic

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Deciphering Phenotypes from Protein Biomarkers for Translational Research with PIPER.

Putty Reddy Sudhir S Alontaga Aileen Y AY Welsh Eric A EA Haura Eric B EB Boyle Theresa A TA Eschrich Steven A SA Koomen John M JM

Journal of proteome research 20230512 6

Liquid chromatography-multiple reaction monitoring mass spectrometry (LC-MRM) has widespread clinical use for detection of inborn errors of metabolism, therapeutic drug monitoring, and numerous other applications. This technique detects proteolytic peptides as surrogates for protein biomarker expression, mutation, and post-translational modification in individual clinical assays and in cancer research with highly multiplexed quantitation across biological pathways. LC-MRM for protein biomarkers ...[more]

PMID: 37171072

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