Proteomics

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Proteomic characterization of extracellular matrix (ECM) produced by human fibroblast activation protein (FAP)-positive pericyte-like cells and glioma cells

ABSTRACT: Fibroblast activation protein (FAP) is upregulated in most cancers and represents an emerging theranostic target in oncology. In glioblastoma (GBM), FAP is predominantly expressed in mesenchymal pericyte-like cells, which contribute to GBM progression by promoting angiogenesis and glioma cell proliferation by soluble mediators. In this work, we show another possible mechanism by which these cells contribute to gliomagenesis. We observed that FAP+ pericyte-like cells residing in the perivascular niche in GBM are associated with elevated levels of the fibrillar extracellular matrix (ECM) proteins collagen I and fibronectin. Using sequential window acquisition of all theoretical mass spectra (SWATH-MS) analysis, we confirmed that the highest levels of collagen I and fibronectin are produced by FAP+ pericyte-like cells. In addition, we observed different expression pattern of basal membrane proteins between FAP+ pericyte-like cells and glioma cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jakub Cervenka  

PROVIDER: PXD045057 | panorama | Tue May 07 00:00:00 BST 2024

REPOSITORIES: PanoramaPublic

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Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination.

Vymola Petr P   Garcia-Borja Elena E   Cervenka Jakub J   Balaziova Eva E   Vymolova Barbora B   Veprkova Jana J   Vodicka Petr P   Skalnikova Helena H   Tomas Robert R   Netuka David D   Busek Petr P   Sedo Aleksi A  

Brain pathology (Zurich, Switzerland) 20240505 6

Gliomagenesis induces profound changes in the composition of the extracellular matrix (ECM) of the brain. In this study, we identified a cellular population responsible for the increased deposition of collagen I and fibronectin in glioblastoma. Elevated levels of the fibrillar proteins collagen I and fibronectin were associated with the expression of fibroblast activation protein (FAP), which is predominantly found in pericyte-like cells in glioblastoma. FAP+ pericyte-like cells were present in  ...[more]

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