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Hs_Hela_LysC_HCD

ABSTRACT: Human hela proteome, LysC HCD

REANALYSIS of: PXD000900

INSTRUMENT(S): QExactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Guo X, Trudgian DC, Lemoff A, Yadavalli S, Mirzaei H

PROVIDER: PAe005072 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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Confetti: a multiprotease map of the HeLa proteome for comprehensive proteomics.

Guo Xiaofeng X Trudgian David C DC Lemoff Andrew A Yadavalli Sivaramakrishna S Mirzaei Hamid H

Molecular & cellular proteomics : MCP 20140402 6

Bottom-up proteomics largely relies on tryptic peptides for protein identification and quantification. Tryptic digestion often provides limited coverage of protein sequence because of issues such as peptide length, ionization efficiency, and post-translational modification colocalization. Unfortunately, a region of interest in a protein, for example, because of proximity to an active site or the presence of important post-translational modifications, may not be covered by tryptic peptides. Detec ...[more]

PMID: 24696503

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