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Hs_CSF_Glyco_Mixed-Mode

ABSTRACT: Human CSF Glyco Mixed Mode

REANALYSIS of: PXD000653

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Guldbrandsen A, Vethe H, Farag Y, Oveland E, Garberg H, Berle M, Myhr KM, Opsahl JA, Barsnes H, Berven FS

PROVIDER: PAe005109 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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In-depth characterization of the cerebrospinal fluid (CSF) proteome displayed through the CSF proteome resource (CSF-PR).

Guldbrandsen Astrid A Vethe Heidrun H Farag Yehia Y Oveland Eystein E Garberg Hilde H Berle Magnus M Myhr Kjell-Morten KM Opsahl Jill A JA Barsnes Harald H Berven Frode S FS

Molecular & cellular proteomics : MCP 20140718 11

In this study, the human cerebrospinal fluid (CSF) proteome was mapped using three different strategies prior to Orbitrap LC-MS/MS analysis: SDS-PAGE and mixed mode reversed phase-anion exchange for mapping the global CSF proteome, and hydrazide-based glycopeptide capture for mapping glycopeptides. A maximal protein set of 3081 proteins (28,811 peptide sequences) was identified, of which 520 were identified as glycoproteins from the glycopeptide enrichment strategy, including 1121 glycopeptides ...[more]

PMID: 25038066

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