Ontology highlight
ABSTRACT:
REANALYSIS of: PXD000293
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Others
SUBMITTER: Albert Heck
PROVIDER: PAe005124 | PeptideAtlas | 2014-12-31
REPOSITORIES: PeptideAtlas
Items per page: 1 - 5 of 8 |
Molecular & cellular proteomics : MCP 20140521 9
Quantitative phosphoproteomics workflows traditionally involve additional sample labeling and fractionation steps for accurate and in-depth analysis. Here we report a high-throughput, straightforward, and comprehensive label-free phosphoproteomics approach using the highly selective, reproducible, and sensitive Ti(4+)-IMAC phosphopeptide enrichment method. We demonstrate the applicability of this approach by monitoring the phosphoproteome dynamics of Jurkat T cells stimulated by prostaglandin E2 ...[more]