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Mm_Microglia_highPH

ABSTRACT: Mouse BV-2 microglial proteome

REANALYSIS of: PXD000168

INSTRUMENT(S): QExactive

ORGANISM(S): Mus Musculus

SUBMITTER: Han D, Moon S, Kim Y, Kim J, Jin J, Kim Y

PROVIDER: PAe005146 | PeptideAtlas | 2013-12-31

REPOSITORIES: PeptideAtlas

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Publications

In-depth proteomic analysis of mouse microglia using a combination of FASP and StageTip-based, high pH, reversed-phase fractionation.

Han Dohyun D Moon Sungyoon S Kim Yikwon Y Kim Jihye J Jin Jonghwa J Kim Youngsoo Y

Proteomics 20130913 20

Microglia are major immune cells in the central nervous system. A characterization of microglia proteome would facilitate on the study of microglial functions in association with various neurodegenerative diseases. To build a reference proteome, we established a BV-2 microglial proteome to a depth of 5494 unique protein groups using a novel strategy that combined FASP, StageTip-based high pH fractionation, and high-resolution MS quickly and cost efficiently. By bioinformatics analysis, the BV-2 ...[more]

PMID: 23943505

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