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Hs_Lung_Phospho_SequentialIMAC_cell

ABSTRACT: Sequential Phosphoproteomic Enrichment by Complementary MD-IMAC, Raji cell line

REANALYSIS of: PXD000314

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Others

SUBMITTER: Tsai CF, et al.

PROVIDER: PAe005320 | PeptideAtlas | 2014-12-31

REPOSITORIES: PeptideAtlas

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Sequential phosphoproteomic enrichment through complementary metal-directed immobilized metal ion affinity chromatography.

Tsai Chia-Feng CF Hsu Chuan-Chih CC Hung Jo-Nan JN Wang Yi-Ting YT Choong Wai-Kok WK Zeng Ming-Yao MY Lin Pei-Yi PY Hong Ruo-Wei RW Sung Ting-Yi TY Chen Yu-Ju YJ

Analytical chemistry 20131217 1

Methodologies to enrich heterogeneous types of phosphopeptides are critical for comprehensive mapping of the under-explored phosphoproteome. Taking advantage of the distinct binding affinities of Ga(3+) and Fe(3+) for phosphopeptides, we designed a metal-directed immobilized metal ion affinity chromatography for the sequential enrichment of phosphopeptides. In Raji B cells, the sequential Ga(3+)-Fe(3+)-immobilized metal affinity chromatography (IMAC) strategy displayed a 1.5-3.5-fold superior ph ...[more]

PMID: 24313913

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