Project description:To explore the influence of gene expression in different time sequences and pathway changes in spinal lumbar segment after SCI To investigate the key transcriptional regulatory factors regulating spinal lumbar axon regeneration after SCI We then performed gene expression profiling analysis using data obtained from RNA-seq of spinal lumbar (L3-5) samples of SCI model at four time points.
Project description:We assessed the transcriptome within lumbar spinal cord tissue of wild-type Lewis rats and attractin-mutant rats (LEWzizi; LEW.SD-Atrn zi/zi).
Project description:Given the leading cause of disability worldwide, low back pain (LBP) is recognized as a pivotal socio-economic challenge to the aging population, which is importantly attributed to intervertebral disc degeneration (IVDD), a highly prevalent affliction of aging. Elastic nucleus pulposus (NP) tissue is essential for maintenance of IVD structural and functional integrity. Native NP cells exhibit crucial functions for regulating extracellular matrix homeostasis, constructing an accommodating biomechanical environment and maintaining the gelatinous property of NP tissue. Mechanical stresses, including compulsion position and aberrant mechanical loading, are regarded as etiological factors of IVDD, and to further investigate the effect of mechanical exercise during the degeneration of IVDs, we surgically resected the lumbar 4th-lumbar 5th (L4-L5) spinous processes of the rat spine along with the supraspinous and interspinous ligaments to induce lumbar surgical instability (LSI). Additionally, we applied RNA sequencing technology to describe the degeneration-associated molecular atlas of the LSI model during exercise, and found that exercise accelerated the degenerative process of IVDs from LSI, which is involved in cytosolic DNA sensing-mediated inflammatory activation, similar to the degenerative patterns of the clinical IVDD process.
Project description:This study includes spatial transcriptomics on the human lumbar spinal cord using the 10x Genomics Visium platform. Frozen sections of spinal cord were placed on Visium slide arrays and processed using the 10x Genomics workflow, followed by alignment and quantification using the spaceranger package.
Project description:Single cell RNAseq was performed on naïve adult mouse lumbar dorsal root ganglia (DRG) cells. Neuronal and non-neuronal cell populations were identified.
Project description:Exercise accelerated the degeneration of lumbar IVDs from surgical lumbar instability, similar to the clinical degeneration process
Project description:The present study aimed to investigate differentially expressed genes in whole blood obtained from patients with lumbar disc prolapse and healthy volunteers. A total of 8 patients with lumbar disc prolapse and 8 healthy volunteers were recruited. An Agilent SurePrint G3 human gene expression microarray 8x60 K was used to perform the microarray analyses.
Project description:Interventions: Experimental group:Ultrasound-guided paravertebral nerve block combined with lumbar quadratus muscle block;Control group:Bilateral lumbar quadratus block guided by ultrasound
Primary outcome(s): VAS score at rest and exercise at 2, 4, 6, 12, 24 and 48 hours after operation
Study Design: Parallel