Proteomics

Dataset Information

0

Strong cation exchange combined with COFRADIC methionine proteome of profilerating human MAPC


ABSTRACT: Not available

INSTRUMENT(S): Bruker Daltonics flex series, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Multipotent Stem Cell, Stem Cell, Bone Marrow

SUBMITTER: Lennart Martens  

PROVIDER: PRD000011 | Pride | 2006-04-18

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
PRIDE_Exp_Complete_Ac_1642.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_1642.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_1642.xml.gz Xml
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Publications

Four stage liquid chromatographic selection of methionyl peptides for peptide-centric proteome analysis: the proteome of human multipotent adult progenitor cells.

Gevaert Kris K   Pinxteren Jef J   Demol Hans H   Hugelier Koen K   Staes An A   Van Damme Jozef J   Martens Lennart L   Vandekerckhove Joël J  

Journal of proteome research 20060601 6


Serial application of strong cation-exchange and diagonal reversed-phase chromatography selecting methionyl peptides by stepwise shifting them from their reduced to their sulfoxide and sulfone forms generates a four-stage fractionation system, allowing high coverage analysis of complex proteome digests by LC-MALDI-MS/MS. Application to the proteome of a human multipotent adult progenitor cell line (MAPC) identified 2151 proteins with high confidence as on average four MS/MS-spectra were linked t  ...[more]

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