Proteomics

Dataset Information

0

Effects of subminimum inhibitory concentrations of antibiotics on the Pasteurella multocida proteome.


ABSTRACT: Not available

INSTRUMENT(S): instrument model, LCQ Deca XP Plus

ORGANISM(S): Pasteurella Multocida Subsp. Multocida Str. Pm70

SUBMITTER: DAVE THORNEYCROFT  

PROVIDER: PRD000014 | Pride | 2006-04-06

REPOSITORIES: Pride

Dataset's files

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Publications

Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.

Gygi S P SP   Rist B B   Gerber S A SA   Turecek F F   Gelb M H MH   Aebersold R R  

Nature biotechnology 19991001 10


We describe an approach for the accurate quantification and concurrent sequence identification of the individual proteins within complex mixtures. The method is based on a class of new chemical reagents termed isotope-coded affinity tags (ICATs) and tandem mass spectrometry. Using this strategy, we compared protein expression in the yeast Saccharomyces cerevisiae, using either ethanol or galactose as a carbon source. The measured differences in protein expression correlated with known yeast meta  ...[more]

Publication: 1/3

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