Ontology highlight
ABSTRACT:
INSTRUMENT(S): instrument model, LCQ Deca XP Plus
ORGANISM(S): Pasteurella Multocida Subsp. Multocida Str. Pm70
SUBMITTER: DAVE THORNEYCROFT
PROVIDER: PRD000014 | Pride | 2006-04-06
REPOSITORIES: Pride
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Nature biotechnology 19991001 10
We describe an approach for the accurate quantification and concurrent sequence identification of the individual proteins within complex mixtures. The method is based on a class of new chemical reagents termed isotope-coded affinity tags (ICATs) and tandem mass spectrometry. Using this strategy, we compared protein expression in the yeast Saccharomyces cerevisiae, using either ethanol or galactose as a carbon source. The measured differences in protein expression correlated with known yeast meta ...[more]