Proteomics

Dataset Information

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HUPO Brain Proteome Project - Pilot study of the Human Proteome Organisation Brain Proteome Project: applying different 2-DE techniques to monitor proteomic changes during murine brain development


ABSTRACT: Not available

INSTRUMENT(S): Bruker Daltonics flex series, instrument model, LCQ Classic, 4700 Proteomics Analyzer

ORGANISM(S): Homo Sapiens (human) Mus

TISSUE(S): Brain

SUBMITTER: DAVE THORNEYCROFT  

PROVIDER: PRD000018 | Pride | 2006-11-30

REPOSITORIES: Pride

Dataset's files

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Publications

Gel-free analysis of the human brain proteome: application of liquid chromatography and mass spectrometry on biopsy and autopsy samples.

Dumont Debora D   Noben Jean-Paul JP   Verhaert Peter P   Stinissen Piet P   Robben Johan J  

Proteomics 20060901 18


This paper reports on the findings of the Biomedical Research Institute, as one of the participants in the pilot study of the HUPO Brain Proteome Project. A biopsy and autopsy study sample derived from human brain was distributed among the participants for proteomic analysis. In our laboratory, attention was focused on protein identification using the bottom-up shotgun approach. Protein extracts derived from both samples were trypsinized and analyzed separately by 2-D LC and MS. In a complementa  ...[more]

Publication: 1/16

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