Dataset Information

Straightforward peptide sequencing by MALDI-MS/MS using a Lys-N metallopeptidase - Boersema et al. 2008

ABSTRACT: Not available

INSTRUMENT(S): instrument model

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Paul Boersema

PROVIDER: PRD000052 | Pride | 2008-12-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_3380.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_3380.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_3380.xml.gz	Xml

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Publications

Straightforward and de novo peptide sequencing by MALDI-MS/MS using a Lys-N metalloendopeptidase.

Boersema Paul J PJ Taouatas Nadia N Altelaar A F Maarten AF Gouw Joost W JW Ross Philip L PL Pappin Darryl J DJ Heck Albert J R AJ Mohammed Shabaz S

Molecular & cellular proteomics : MCP 20081129 4

In this work, we explore the potential of the metalloendopeptidase Lys-N for MALDI-MS/MS proteomics applications. Initially we digested a HEK293 cellular lysate with Lys-N and, for comparison, in parallel with the protease Lys-C. The resulting peptides were separated by strong cation exchange to enrich and isolate peptides containing a single N-terminal lysine. MALDI-MS/MS analysis of these peptides yielded CID spectra with clear and often complete sequence ladders of b-ions. To test the applica ...[more]

PMID: 19043101

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