Proteomics

Dataset Information

0

Projects of the Clinical Proteomics Laboratories at the Medical University of Vienna (CPL/MUW)


ABSTRACT: Not available

INSTRUMENT(S): instrument model, Agilent instrument model

ORGANISM(S): Rattus Norvegicus (rat) Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Kupffer Cell, Mononuclear Cell, Blood Plasma, Monocyte, Leukocyte, Hepg2 Cell, Liver, Melanocyte, Blood, Melanoma Cell Line, Erythrocyte, Platelet, Primary Cell, Hep-3b Cell, Hepatocyte, T Cell, Dendritic Cell, Keratinocyte, Skin, Neutrophil, Fibroblast, Bone Marrow, Blood Vessel Endothelial Cell

SUBMITTER: Christopher Gerner  

PROVIDER: PRD000053 | Pride | 2012-05-02

REPOSITORIES: Pride

Dataset's files

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Publications

Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B.

Slany Astrid A   Haudek Verena J VJ   Zwickl Hannes H   Gundacker Nina C NC   Grusch Michael M   Weiss Thomas S TS   Seir Katja K   Rodgarkia-Dara Chantal C   Hellerbrand Claus C   Gerner Christopher C  

Journal of proteome research 20100101 1


Hepatocytes are known to express a large number of characteristic proteins. Transformed and cultured hepatocytes only partially maintain functional cell differentiation characteristics, which can be assessed by proteome profiling. Here, we applied 2D-PAGE analysis in addition to shotgun proteomics to assess the functional cell state of primary human hepatocytes (PHH), HepG2 and Hep3B cells. Out of a total of 1995 proteins identified in the cytoplasm of these cells, we filtered 107 proteins which  ...[more]

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