Online automated zebrafish phosphoproteomics; From large scale analysis down to a single embryo
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap, instrument model
ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)
TISSUE(S): Embryo
SUBMITTER: 
Phil Jones
PROVIDER: PRD000054 | Pride | 2008-07-01
REPOSITORIES: Pride
Dataset's files
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Publications
Online automated in vivo zebrafish phosphoproteomics: from large-scale analysis down to a single embryo.
Lemeer Simone S Pinkse Martijn W H MW Mohammed Shabaz S van Breukelen Bas B den Hertog Jeroen J Slijper Monique M Heck Albert J R AJ
Journal of proteome research 20080229 4
In the developing embryo, as in many other biological processes, complex signaling pathways are under tight control of reversible phosphorylation, guiding cell proliferation, differentiation, and growth. Therefore the large-scale identification of signaling proteins and their post-translational modifications is crucial to understand the proteome biology of the developing zebrafish embryo. Here, we used an automated, robust, and sensitive online TiO 2-based LC-MS/MS setup to enrich for phosphoryl ...[more]
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