Blue native electrophoresis combined with stable isotope labeling shows that Saccharomyces cerevisiae mitochondrial respiratory chain supercomplexes are still present under anaerobic conditions
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ FT, instrument model
ORGANISM(S): Saccharomyces Cerevisiae (strain Yjm789) (baker's Yeast)
SUBMITTER: 
Andreas Helbig
PROVIDER: PRD000087 | Pride | 2009-07-28
REPOSITORIES: Pride
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Publications
A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions.
Helbig Andreas O AO de Groot Marco J L MJ van Gestel Renske A RA Mohammed Shabaz S de Hulster Erik A F EA Luttik Marijke A H MA Daran-Lapujade Pascale P Pronk Jack T JT Heck Albert J R AJ Slijper Monique M
Proteomics 20091001 20
To investigate the effect of anaerobiosis on the Saccharomyces cerevisiae mitochondrial proteome and the formation of respiratory chain and other protein complexes, we analyzed mitochondrial protein extracts that were enriched from lysates of aerobic and anaerobic steady-state chemostat cultures. We chose an innovative approach in which native mitochondrial membrane protein complexes were separated by 1-D blue native PAGE, which was combined with quantitative analysis of each complex subunit usi ...[more]
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