Proteomics

Dataset Information

0

C. elegans genome annotation project


ABSTRACT: Not available

INSTRUMENT(S): instrument model, LTQ

ORGANISM(S): Caenorhabditis Elegans

SUBMITTER: Gennifer Merrihew  

PROVIDER: PRD000122 | Pride | 2009-12-01

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
PRIDE_Exp_Complete_Ac_9776.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_9776.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_9776.xml.gz Xml
Items per page:
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Publications

Use of shotgun proteomics for the identification, confirmation, and correction of C. elegans gene annotations.

Merrihew Gennifer E GE   Davis Colleen C   Ewing Brent B   Williams Gary G   Käll Lukas L   Frewen Barbara E BE   Noble William Stafford WS   Green Phil P   Thomas James H JH   MacCoss Michael J MJ  

Genome research 20080724 10


We describe a general mass spectrometry-based approach for gene annotation of any organism and demonstrate its effectiveness using the nematode Caenorhabditis elegans. We detected 6779 C. elegans proteins (67,047 peptides), including 384 that, although annotated in WormBase WS150, lacked cDNA or other prior experimental support. We also identified 429 new coding sequences that were unannotated in WS150. Nearly half (192/429) of the new coding sequences were confirmed with RT-PCR data. Thirty-thr  ...[more]

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