Dataset Information

Mouse Stem Cell Database (R1) - Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches

ABSTRACT: Digets of unfractionated whole cell lysate

INSTRUMENT(S): LTQ Orbitrap, instrument model, LTQ

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell

SUBMITTER: Rebekah Gundry

PROVIDER: PRD000194 | Pride | 2012-03-04

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_11364.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_11364.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_11364.xml.gz	Xml
	PRIDE_Exp_Complete_Ac_11365.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_11365.pride.mztab.gz	Mztab

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Publications

Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches.

Gundry Rebekah L RL Tchernyshyov Irina I Sheng Shijun S Tarasova Yelena Y Raginski Kimberly K Boheler Kenneth R KR Van Eyk Jennifer E JE

Proteomics 20100701 14

The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection o ...[more]

PMID: 20512790

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Project description:Stem cells are at the forefront of current regenerative and biomedical research. Thus, there exists an imperative and urgent need to understand the mechanisms that drive stem cell function in order to exploit their use as a therapeutic tool. Amino acids are potent inducers of signaling cascades that drive stem cell proliferation and differentiation. With a focus on mouse embryonic stem (mES) cells, Threonine (Thr) is the only amino acid required in culture media for mES cell proliferation. Current research associates this need for Thr with threonine dehydrogenase (TDH), which catabolizes Thr to glycine and acetyl-CoA in mES cells. This theory depends, in part, on the ability of 3- hydroxynorvaline (3-HNV) to inhibit both TDH and mES cell proliferation. However, the concentration of 3-HNV needed to inhibit mES cell proliferation is more than an order of magnitude less than its apparent Ki for TDH inhibition. Additionally, 3-HNV inhibits human embryonic stem (hES) cell proliferation, but hES cells do not express a functional tdh gene. Such findings indicate another mechanism for Thr stimulated mES and hES cell proliferation. Since amino acid transporters may be inducers of signaling cascades, we characterized the Thr transport systems in mES cells. We found that there is a Na(+)-dependent and a Na(+)-independent component of substrate-saturable transport, with the Na(+)-dependent component predominating. We also found that of 20 amino acids tested, the amino acids that were the strongest inhibitors of the Na(+)-dependent component of radiolabeled Thr transport were Ser, Cys, 4-OH-Pro, Asn, Met, and non-radiolabeled Thr itself. Such findings are consistent with characteristics of the ASC transport system, suggesting that this ASC system is responsible for the majority of Thr transport in mES cells. We confirmed expression of mRNA encoding the ASC system transporters, ASCT1 and ASCT2, in mES cells using RT-PCR. In conclusion, mES cells likely express at least three transporters of Thr; at least two Na(+)-dependent transporters and one Na(+)-independent one.