Proteomics

Dataset Information

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Mouse Stem Cell Database (R1) - Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches


ABSTRACT: Digets of unfractionated whole cell lysate

INSTRUMENT(S): LTQ Orbitrap, instrument model, LTQ

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Stem Cell

SUBMITTER: Rebekah Gundry  

PROVIDER: PRD000194 | Pride | 2012-03-04

REPOSITORIES: Pride

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Publications

Expanding the mouse embryonic stem cell proteome: combining three proteomic approaches.

Gundry Rebekah L RL   Tchernyshyov Irina I   Sheng Shijun S   Tarasova Yelena Y   Raginski Kimberly K   Boheler Kenneth R KR   Van Eyk Jennifer E JE  

Proteomics 20100701 14


The current study used three different proteomic strategies, which differed by their extent of intact protein separation, to examine the proteome of a pluripotent mouse embryonic stem cell line, R1. Proteins from whole-cell lysates were subjected either to 2-D-LC, or 1-DE, or were unfractionated prior to enzymatic digestion and subsequent analysis by MS. The results yielded 1895 identified non-redundant proteins and, for 128 of these, the specific isoform could be determined based on detection o  ...[more]

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