Proteomics

Dataset Information

0

FFPE - Formalin-fixed paraffin-embedded (FFPE) proteome analysis using gel-free and gel-based proteomics


ABSTRACT: protein extraction from FFPE tissue comparison

INSTRUMENT(S): LTQ Orbitrap, instrument model, 4700 Proteomics Analyzer

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Hakan Sarioglu  

PROVIDER: PRD000234 | Pride | 2011-05-05

REPOSITORIES: Pride

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Publications

Formalin-fixed paraffin-embedded (FFPE) proteome analysis using gel-free and gel-based proteomics.

Azimzadeh Omid O   Barjaktarovic Zarko Z   Aubele Michaela M   Calzada-Wack Julia J   Sarioglu Hakan H   Atkinson Michael J MJ   Tapio Soile S  

Journal of proteome research 20100901 9


Formalin-fixed paraffin-embedded (FFPE) tissue has recently gained interest as an alternative to fresh/frozen tissue for retrospective protein biomarker discovery. However, during the fixation process, proteins undergo degradation and cross-linking, making conventional protein analysis technologies problematic. In this study, we have compared several extraction and separation methods for the analysis of proteins in FFPE tissues. Incubation of tissue sections at high temperature with a novel extr  ...[more]

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