Proteomics

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Arabidopsis drought - A reciprocal 15N-labeling proteomic analysis of expanding Arabidopsis leaves subjected to osmotic stress indicates importance of mitochondria in preserving plastid functions


ABSTRACT: Mannitol induced drought stress, metabolic labeling experiment 14N control vs 15N mannitol induced drought stress, 0mM mannitol control experiment, LCMSMS of gel slice10 of 20, 1st of 2 replicas, 14 N peptide searching

INSTRUMENT(S): instrument model

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

SUBMITTER: Bart Devreese  

PROVIDER: PRD000246 | Pride | 2010-12-23

REPOSITORIES: Pride

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A reciprocal 15N-labeling proteomic analysis of expanding Arabidopsis leaves subjected to osmotic stress indicates importance of mitochondria in preserving plastid functions.

Skirycz Aleksandra A   Memmi Samy S   De Bodt Stefanie S   Maleux Katrien K   Obata Toshihiro T   Fernie Alisdair R AR   Devreese Bart B   Inzé Dirk D  

Journal of proteome research 20110125 3


Plants respond to environmental stress by dynamically reprogramming their growth. Whereas stress onset is accompanied by rapid growth inhibition leading to smaller organs, growth will recover and adapt once the stress conditions become stable and do no threaten plant survival. Here, adaptation of growing Arabidopsis thaliana leaves to mild and prolonged osmotic stress was investigated by means of a complete metabolic labeling strategy with the (15)N-stable isotope as a complement to a previously  ...[more]

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