Team 17 Sanger - Shotgun proteomics aids discovery of novel protein-coding genes, alternative splicing, and "resurrected" pseudogenes in the mouse genome
Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ FT, instrument model
ORGANISM(S): Mus Musculus (mouse)
TISSUE(S): Epiblast Cell
SUBMITTER: 
James Wright
PROVIDER: PRD000328 | Pride | 2012-10-17
REPOSITORIES: Pride
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Shotgun proteomics aids discovery of novel protein-coding genes, alternative splicing, and "resurrected" pseudogenes in the mouse genome.
Brosch Markus M Saunders Gary I GI Frankish Adam A Collins Mark O MO Yu Lu L Wright James J Verstraten Ruth R Adams David J DJ Harrow Jennifer J Choudhary Jyoti S JS Hubbard Tim T
Genome research 20110401 5
Recent advances in proteomic mass spectrometry (MS) offer the chance to marry high-throughput peptide sequencing to transcript models, allowing the validation, refinement, and identification of new protein-coding loci. We present a novel pipeline that integrates highly sensitive and statistically robust peptide spectrum matching with genome-wide protein-coding predictions to perform large-scale gene validation and discovery in the mouse genome for the first time. In searching an excess of 10 mil ...[more]
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