Dataset Information

Horse articular cartilage explant secretome

ABSTRACT: proteins secreted during 5 day culture of articular cartilage explants

INSTRUMENT(S): instrument model, Bruker Daltonics amaZon series

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Tissue Culture

SUBMITTER: Susan Liddell

PROVIDER: PRD000359 | Pride | 2012-03-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	PRIDE_Exp_Complete_Ac_15852.pride.mgf.gz	Mgf
	PRIDE_Exp_Complete_Ac_15852.pride.mztab.gz	Mztab
	PRIDE_Exp_Complete_Ac_15852.xml.gz	Xml

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Publications

High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation.

Clutterbuck Abigail L AL Smith Julia R JR Allaway David D Harris Pat P Liddell Susan S Mobasheri Ali A

Journal of proteomics 20110224 5

This study employed a targeted high-throughput proteomic approach to identify the major proteins present in the secretome of articular cartilage. Explants from equine metacarpophalangeal joints were incubated alone or with interleukin-1beta (IL-1β, 10ng/ml), with or without carprofen, a non-steroidal anti-inflammatory drug, for six days. After tryptic digestion of culture medium supernatants, resulting peptides were separated by HPLC and detected in a Bruker amaZon ion trap instrument. The five ...[more]

PMID: 21354348

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