Proteomics

Dataset Information

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Methionine sulfoxide - Redox proteomics of protein-bound methionine oxidation

ABSTRACT: This project contains the COFRADIC data of two experimental set-ups (swapped labeling) in which methionine sulfoxides were identified in Jurkat cells stressed with hydrogen peroxide. We used a SILAC set-up in which cells were labeled with 12C5 methionine or with 13C5 methionine. One of the set-ups was treated with hydrogen peroxide, the other with H2O. Following lysis proteins were mixed in a 1/1 ratio. We then performed our methionine sulfoxide validation protocol onto the sample identifying methionine sulfoxides in the stressed Jurkat cells.

INSTRUMENT(S): LTQ Orbitrap, instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

SUBMITTER: Bart Ghesquiere  

PROVIDER: PRD000395 | Pride | 2011-03-18

REPOSITORIES: pride

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Dataset's files

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Action DRS
PRIDE_Exp_Complete_Ac_16645.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_16645.pride.mztab.gz Mztab
PRIDE_Exp_Complete_Ac_16645.xml.gz Xml
PRIDE_Exp_Complete_Ac_16646.pride.mgf.gz Mgf
PRIDE_Exp_Complete_Ac_16646.pride.mztab.gz Mztab
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Publications

We here present a new method to measure the degree of protein-bound methionine sulfoxide formation at a proteome-wide scale. In human Jurkat cells that were stressed with hydrogen peroxide, over 2000 oxidation-sensitive methionines in more than 1600 different proteins were mapped and their extent of oxidation was quantified. Meta-analysis of the sequences surrounding the oxidized methionine residues revealed a high preference for neighboring polar residues. Using synthetic methionine sulfoxide c  ...[more]

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